Schematic overview of the cellular and molecular mechanisms involved in the cancer progression, including the proposed cellular and molecular mechanisms in cancer cells trajectory. AT1: alveolar type 1 cells; AT2: alveolar type 2 cells; AAH: atypical adenomatous hyperplasia; AIS: adenocarcinoma in situ; MIA: minimally invasive adenocarcinoma; IA: invasive adenocarcinoma; EMT: epithelial-mesenchymal transition

Home

Download

Statistics

Help

Contact

	Gene summary
	Malignant transformation analysis
	Malignant transformation related pathway analysis
	Cell-cell communication analysis
	Single-cell gene regulatory network inference analysis
	Somatic mutation of malignant transformation related genes
	Related drugs of malignant transformation related genes

Gene: ANPEP

Gene summary for ANPEP

Gene summary.

Gene information	Species	Human
	Gene symbol	ANPEP
	Gene ID	290
	Gene name	alanyl aminopeptidase, membrane
	Gene Alias	APN
	Cytomap	15q26.1
	Gene Type	protein-coding
	GO ID	GO:0001525
	UniProtAcc	A0A024RC61

Top

Malignant transformation analysis

Identification of the aberrant gene expression in precancerous and cancerous lesions by comparing the gene expression of stem-like cells in diseased tissues with normal stem cells

Malignant transformation involving gene list.

Entrez ID	Symbol	Replicates	Species	Organ	Tissue	Adj P-value	Log2FC	Malignancy
290	ANPEP	GSM4909299	Human	Breast	IDC	5.88e-04	2.28e-01	0.035
290	ANPEP	GSM4909308	Human	Breast	IDC	2.82e-02	-1.20e-01	0.158
290	ANPEP	brca10	Human	Breast	Precancer	4.07e-02	-1.20e-01	-0.0029
290	ANPEP	DCIS2	Human	Breast	DCIS	5.45e-57	5.58e-01	0.0085
290	ANPEP	HTA11_3410_2000001011	Human	Colorectum	AD	4.14e-11	-3.49e-01	0.0155
290	ANPEP	HTA11_2487_2000001011	Human	Colorectum	SER	2.05e-17	2.39e+00	-0.1808
290	ANPEP	HTA11_1938_2000001011	Human	Colorectum	AD	1.07e-38	3.02e+00	-0.0811
290	ANPEP	HTA11_2112_2000001011	Human	Colorectum	SER	3.96e-03	2.01e+00	-0.2196
290	ANPEP	HTA11_83_2000001011	Human	Colorectum	SER	2.44e-14	2.52e+00	-0.1526
290	ANPEP	HTA11_866_3004761011	Human	Colorectum	AD	2.09e-09	-3.48e-01	0.096
290	ANPEP	HTA11_7696_3000711011	Human	Colorectum	AD	1.68e-10	-3.46e-01	0.0674
290	ANPEP	HTA11_6818_2000001021	Human	Colorectum	AD	7.67e-03	-3.40e-01	0.0588
290	ANPEP	HTA11_99999970781_79442	Human	Colorectum	MSS	1.58e-13	-3.54e-01	0.294
290	ANPEP	HTA11_99999965104_69814	Human	Colorectum	MSS	9.31e-03	-3.29e-01	0.281
290	ANPEP	HTA11_99999971662_82457	Human	Colorectum	MSS	3.73e-11	-3.50e-01	0.3859
290	ANPEP	HTA11_99999974143_84620	Human	Colorectum	MSS	5.72e-06	-3.14e-01	0.3005
290	ANPEP	F007	Human	Colorectum	FAP	7.01e-05	-3.29e-01	0.1176
290	ANPEP	A002-C-010	Human	Colorectum	FAP	7.06e-09	-3.46e-01	0.242
290	ANPEP	A001-C-207	Human	Colorectum	FAP	5.27e-05	-2.85e-01	0.1278
290	ANPEP	A015-C-203	Human	Colorectum	FAP	1.61e-05	-2.38e-01	-0.1294

Page: 1 2 3 4 5

Transcriptomic changes along malignancy continuum.

Tissue	Expression Dynamics	Abbreviation
Breast		IDC: Invasive ductal carcinoma
		DCIS: Ductal carcinoma in situ
		Precancer(BRCA1-mut): Precancerous lesion from BRCA1 mutation carriers
Colorectum (GSE201348)		FAP: Familial adenomatous polyposis
		CRC: Colorectal cancer
Colorectum (HTA11)		AD: Adenomas
		SER: Sessile serrated lesions
		MSI-H: Microsatellite-high colorectal cancer
		MSS: Microsatellite stable colorectal cancer
GC		CAG: Chronic atrophic gastritis
		CAG with IM: Chronic atrophic gastritis with intestinal metaplasia
		CSG: Chronic superficial gastritis
		GC: Gastric cancer
		SIM: Severe intestinal metaplasia
		WIM: Wild intestinal metaplasia
Liver		HCC: Hepatocellular carcinoma
		NAFLD: Non-alcoholic fatty liver disease
Prostate		BPH: Benign Prostatic Hyperplasia
Thyroid		ATC: Anaplastic thyroid cancer
		HT: Hashimoto's thyroiditis
		PTC: Papillary thyroid cancer

∗log2FC in expression of this searched gene in stem-like cells from each diseased tissue sample relative to stem-like cells in normal samples in each tissue plotted against the malignancy continuum. Samples are colored based on if they are from different disease stage.

Top

Malignant transformation related pathway analysis

Find out the enriched GO biological processes and KEGG pathways involved in transition from healthy to precancer to cancer

Figure of enriched GO biological processes.

Tissue	Disease Stage	Enriched GO biological Processes
Colorectum	AD
Colorectum	SER
Colorectum	MSS
Colorectum	MSI-H
Colorectum	FAP

∗Top 15 enriched GO BP terms are showed in the bar plot of each disease state in each tissue. Each row represents a significant GO biological process which is colored according to the -log10(p.adjust).

Page: 1 2 3 4 5 6 7 8 9

Enriched GO biological processes.

GO ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	Count
GO:00160329	Breast	Precancer	viral process	58/1080	415/18723	3.42e-10	3.16e-08	58
GO:00190589	Breast	Precancer	viral life cycle	47/1080	317/18723	2.42e-09	1.99e-07	47
GO:00444039	Breast	Precancer	biological process involved in symbiotic interaction	42/1080	290/18723	3.40e-08	2.11e-06	42
GO:00521269	Breast	Precancer	movement in host environment	29/1080	175/18723	2.59e-07	1.28e-05	29
GO:00517019	Breast	Precancer	biological process involved in interaction with host	31/1080	203/18723	6.52e-07	2.71e-05	31
GO:00444099	Breast	Precancer	entry into host	25/1080	151/18723	1.74e-06	6.19e-05	25
GO:00467189	Breast	Precancer	viral entry into host cell	24/1080	144/18723	2.46e-06	8.35e-05	24
GO:001603214	Breast	IDC	viral process	75/1434	415/18723	1.98e-12	4.02e-10	75
GO:001905814	Breast	IDC	viral life cycle	61/1434	317/18723	1.61e-11	2.77e-09	61
GO:004440314	Breast	IDC	biological process involved in symbiotic interaction	52/1434	290/18723	6.55e-09	5.03e-07	52
GO:005212614	Breast	IDC	movement in host environment	32/1434	175/18723	3.33e-06	1.22e-04	32
GO:005170114	Breast	IDC	biological process involved in interaction with host	35/1434	203/18723	4.72e-06	1.63e-04	35
GO:004440914	Breast	IDC	entry into host	28/1434	151/18723	1.02e-05	2.82e-04	28
GO:004671814	Breast	IDC	viral entry into host cell	27/1434	144/18723	1.18e-05	3.14e-04	27
GO:001603224	Breast	DCIS	viral process	73/1390	415/18723	3.40e-12	6.88e-10	73
GO:001905824	Breast	DCIS	viral life cycle	59/1390	317/18723	4.05e-11	5.74e-09	59
GO:004440323	Breast	DCIS	biological process involved in symbiotic interaction	49/1390	290/18723	4.70e-08	2.89e-06	49
GO:005212624	Breast	DCIS	movement in host environment	31/1390	175/18723	4.93e-06	1.45e-04	31
GO:005170124	Breast	DCIS	biological process involved in interaction with host	34/1390	203/18723	6.30e-06	1.78e-04	34
GO:004440924	Breast	DCIS	entry into host	27/1390	151/18723	1.65e-05	4.00e-04	27

Page: 1 2 3 4 5 6

Enriched KEGG pathways.

Pathway ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	qvalue	Count
hsa004809	Liver	Cirrhotic	Glutathione metabolism	30/2530	57/8465	2.63e-04	1.72e-03	1.06e-03	30
hsa0048012	Liver	Cirrhotic	Glutathione metabolism	30/2530	57/8465	2.63e-04	1.72e-03	1.06e-03	30
hsa0048022	Liver	HCC	Glutathione metabolism	42/4020	57/8465	5.00e-05	2.62e-04	1.46e-04	42
hsa0048032	Liver	HCC	Glutathione metabolism	42/4020	57/8465	5.00e-05	2.62e-04	1.46e-04	42
hsa0048014	Prostate	Tumor	Glutathione metabolism	20/1791	57/8465	1.05e-02	3.33e-02	2.07e-02	20
hsa0048015	Prostate	Tumor	Glutathione metabolism	20/1791	57/8465	1.05e-02	3.33e-02	2.07e-02	20

Page: 1

Top

Cell-cell communication analysis

Identification of potential cell-cell interactions between two cell types and their ligand-receptor pairs for different disease states

Ligand

Receptor

LRpair

Pathway

Tissue

Disease Stage

Page: 1

Top

Single-cell gene regulatory network inference analysis

Find out the significant the regulons (TFs) and the target genes of each regulon across cell types for different disease states

Cell Type

Tissue

Disease Stage

Target Gene

RSS

Regulon Activity

∗The dot plots of a searched regulon are shown for all cell subpopulations in each disease state of each tissue based on the regulon specific score inferred using pySCENIC and by calculating the average expression.

Page: 1

Top

Somatic mutation of malignant transformation related genes

Annotation of somatic variants for genes involved in malignant transformation

Hugo Symbol

Variant Class

Variant Classification

dbSNP RS

HGVSc

HGVSp

HGVSp Short

SWISSPROT

BIOTYPE

SIFT

PolyPhen

Tumor Sample Barcode

Tissue

Histology

Sex

Age

Stage

Therapy Types

Drugs

Outcome

ANPEP

SNV

Missense_Mutation

rs141631662

c.1088N>A

p.Arg363Gln

p.R363Q

P15144

protein_coding

tolerated(0.13)

possibly_damaging(0.844)

TCGA-A2-A0CV-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

cytoxan

ANPEP

SNV

Missense_Mutation

c.256N>T

p.Arg86Trp

p.R86W

P15144

protein_coding

deleterious(0.01)

possibly_damaging(0.773)

TCGA-AO-A128-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

doxorubicin

ANPEP

SNV

Missense_Mutation

rs766949181

c.67G>A

p.Val23Met

p.V23M

P15144

protein_coding

deleterious(0.04)

benign(0.441)

TCGA-B6-A0RG-01

Breast

breast invasive carcinoma

Female

<65

I/II

Unknown

ANPEP

SNV

Missense_Mutation

c.422N>A

p.Gly141Asp

p.G141D

P15144

protein_coding

tolerated(0.21)

possibly_damaging(0.606)

TCGA-BH-A0BC-01

Breast

breast invasive carcinoma

Female

<65

III/IV

Chemotherapy

doxorubicin

ANPEP

SNV

Missense_Mutation

rs376373199

c.2831C>T

p.Thr944Met

p.T944M

P15144

protein_coding

deleterious(0)

probably_damaging(0.999)

TCGA-D8-A140-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

doxorubicin+cyclophosphamid

ANPEP

SNV

Missense_Mutation

c.1928N>C

p.Gln643Pro

p.Q643P

P15144

protein_coding

deleterious(0.03)

possibly_damaging(0.625)

TCGA-D8-A27M-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

methotrexate+5

ANPEP

SNV

Missense_Mutation

c.881N>T

p.Ala294Val

p.A294V

P15144

protein_coding

tolerated(0.35)

benign(0.026)

TCGA-EW-A1IZ-01

Breast

breast invasive carcinoma

Female

<65

III/IV

Chemotherapy

taxotere

ANPEP

insertion

In_Frame_Ins

novel

c.2569_2570insGTG

p.Gln857delinsArgGlu

p.Q857delinsRE

P15144

protein_coding

TCGA-A2-A0D1-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Chemotherapy

taxotere

ANPEP

insertion

In_Frame_Ins

novel

c.2568_2569insGGGCACTCCATTCTTCTCCCG

p.Lys856_Gln857insGlyHisSerIleLeuLeuPro

p.K856_Q857insGHSILLP

P15144

protein_coding

TCGA-A2-A0D1-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Chemotherapy

taxotere

ANPEP

deletion

In_Frame_Del

novel

c.2300_2302delAGT

p.Glu767_Cys768delinsGly

p.E767_C768delinsG

P15144

protein_coding

TCGA-A8-A096-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Unknown

Page: 1 2 3 4 5 6 7 8 9 10 11

Top

Related drugs of malignant transformation related genes

Identification of chemicals and drugs interact with genes involved in malignant transfromation

(DGIdb 4.0)

Entrez ID	Symbol	Category	Interaction Types	Drug Claim Name	Drug Name	PMIDs
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME		RECOMBINANT ERYTHROPOIETIN		15957544
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME	inhibitor	CHEMBL2103847	TOSEDOSTAT
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME	inhibitor	348353628
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME		CEPHALOSPORINS		8774083
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME		CEFADROXIL	CEFADROXIL	8774083
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME	inhibitor	315661176
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME		ZINC SULFATE		14568236
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME	inhibitor	348353627
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME	inhibitor	252166766
290	ANPEP	NEUTRAL ZINC METALLOPEPTIDASE, EXTERNAL SIDE OF PLASMA MEMBRANE, DRUGGABLE GENOME, PROTEASE, ENZYME		TRANSFORMING GROWTH FACTOR		15138629

Page: 1 2 3 4