Schematic overview of the cellular and molecular mechanisms involved in the cancer progression, including the proposed cellular and molecular mechanisms in cancer cells trajectory. AT1: alveolar type 1 cells; AT2: alveolar type 2 cells; AAH: atypical adenomatous hyperplasia; AIS: adenocarcinoma in situ; MIA: minimally invasive adenocarcinoma; IA: invasive adenocarcinoma; EMT: epithelial-mesenchymal transition

Home

Download

Statistics

Help

Contact

	Gene summary
	Malignant transformation analysis
	Malignant transformation related pathway analysis
	Cell-cell communication analysis
	Single-cell gene regulatory network inference analysis
	Somatic mutation of malignant transformation related genes
	Related drugs of malignant transformation related genes

Gene: ANAPC4

Gene summary for ANAPC4

Gene summary.

Gene information	Species	Human
	Gene symbol	ANAPC4
	Gene ID	29945
	Gene name	anaphase promoting complex subunit 4
	Gene Alias	APC4
	Cytomap	4p15.2
	Gene Type	protein-coding
	GO ID	GO:0000070
	UniProtAcc	B3KN47

Top

Malignant transformation analysis

Identification of the aberrant gene expression in precancerous and cancerous lesions by comparing the gene expression of stem-like cells in diseased tissues with normal stem cells

Malignant transformation involving gene list.

Entrez ID	Symbol	Replicates	Species	Organ	Tissue	Adj P-value	Log2FC	Malignancy
29945	ANAPC4	AEH-subject3	Human	Endometrium	AEH	1.76e-06	4.01e-01	-0.2576
29945	ANAPC4	AEH-subject4	Human	Endometrium	AEH	1.91e-08	4.85e-01	-0.2657
29945	ANAPC4	EEC-subject3	Human	Endometrium	EEC	1.10e-08	-1.89e-01	-0.2525
29945	ANAPC4	GSM5276934	Human	Endometrium	EEC	2.15e-18	-4.12e-01	-0.0913
29945	ANAPC4	GSM5276935	Human	Endometrium	EEC	3.74e-03	-1.92e-01	-0.123
29945	ANAPC4	GSM5276937	Human	Endometrium	EEC	2.95e-05	-2.72e-01	-0.0897
29945	ANAPC4	GSM6177620_NYU_UCEC1_lib1_lib1	Human	Endometrium	EEC	6.25e-11	-2.91e-01	-0.1869
29945	ANAPC4	GSM6177620_NYU_UCEC1_lib2_lib2	Human	Endometrium	EEC	1.08e-07	-3.41e-01	-0.1875
29945	ANAPC4	GSM6177620_NYU_UCEC1_lib3_lib3	Human	Endometrium	EEC	1.66e-16	-2.22e-01	-0.1883
29945	ANAPC4	GSM6177621_NYU_UCEC2_lib1_lib1	Human	Endometrium	EEC	8.04e-09	9.86e-02	-0.1934
29945	ANAPC4	GSM6177622_NYU_UCEC3_lib1_lib1	Human	Endometrium	EEC	5.33e-16	-3.39e-01	-0.1917
29945	ANAPC4	GSM6177622_NYU_UCEC3_lib2_lib2	Human	Endometrium	EEC	2.16e-17	-3.00e-01	-0.1916
29945	ANAPC4	LZE7T	Human	Esophagus	ESCC	1.87e-03	2.14e-01	0.0667
29945	ANAPC4	LZE24T	Human	Esophagus	ESCC	1.38e-07	1.29e-01	0.0596
29945	ANAPC4	P1T-E	Human	Esophagus	ESCC	4.20e-03	2.95e-01	0.0875
29945	ANAPC4	P2T-E	Human	Esophagus	ESCC	1.06e-22	3.02e-01	0.1177
29945	ANAPC4	P4T-E	Human	Esophagus	ESCC	1.12e-17	2.22e-01	0.1323
29945	ANAPC4	P5T-E	Human	Esophagus	ESCC	6.95e-03	7.63e-02	0.1327
29945	ANAPC4	P8T-E	Human	Esophagus	ESCC	4.53e-13	2.07e-01	0.0889
29945	ANAPC4	P9T-E	Human	Esophagus	ESCC	5.59e-06	5.18e-02	0.1131

Page: 1 2 3 4

Transcriptomic changes along malignancy continuum.

Tissue	Expression Dynamics	Abbreviation
Endometrium		AEH: Atypical endometrial hyperplasia
		EEC: Endometrioid Cancer
Esophagus		ESCC: Esophageal squamous cell carcinoma
		HGIN: High-grade intraepithelial neoplasias
		LGIN: Low-grade intraepithelial neoplasias

∗log2FC in expression of this searched gene in stem-like cells from each diseased tissue sample relative to stem-like cells in normal samples in each tissue plotted against the malignancy continuum. Samples are colored based on if they are from different disease stage.

Top

Malignant transformation related pathway analysis

Find out the enriched GO biological processes and KEGG pathways involved in transition from healthy to precancer to cancer

Figure of enriched GO biological processes.

Tissue	Disease Stage	Enriched GO biological Processes
Colorectum	AD
Colorectum	SER
Colorectum	MSS
Colorectum	MSI-H
Colorectum	FAP

∗Top 15 enriched GO BP terms are showed in the bar plot of each disease state in each tissue. Each row represents a significant GO biological process which is colored according to the -log10(p.adjust).

Page: 1 2 3 4 5 6 7 8 9

Enriched GO biological processes.

GO ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	Count
GO:001049816	Endometrium	AEH	proteasomal protein catabolic process	108/2100	490/18723	2.73e-12	4.67e-10	108
GO:004316116	Endometrium	AEH	proteasome-mediated ubiquitin-dependent protein catabolic process	88/2100	412/18723	1.54e-09	1.36e-07	88
GO:00457877	Endometrium	AEH	positive regulation of cell cycle	56/2100	313/18723	2.73e-04	3.14e-03	56
GO:00002098	Endometrium	AEH	protein polyubiquitination	44/2100	236/18723	4.83e-04	4.97e-03	44
GO:00330446	Endometrium	AEH	regulation of chromosome organization	36/2100	187/18723	8.27e-04	7.73e-03	36
GO:00447726	Endometrium	AEH	mitotic cell cycle phase transition	67/2100	424/18723	2.37e-03	1.78e-02	67
GO:00900686	Endometrium	AEH	positive regulation of cell cycle process	41/2100	236/18723	2.94e-03	2.09e-02	41
GO:00459317	Endometrium	AEH	positive regulation of mitotic cell cycle	23/2100	121/18723	7.76e-03	4.33e-02	23
GO:001049817	Endometrium	EEC	proteasomal protein catabolic process	112/2168	490/18723	6.99e-13	1.40e-10	112
GO:004316117	Endometrium	EEC	proteasome-mediated ubiquitin-dependent protein catabolic process	92/2168	412/18723	3.01e-10	3.23e-08	92
GO:004578713	Endometrium	EEC	positive regulation of cell cycle	59/2168	313/18723	1.04e-04	1.44e-03	59
GO:000020913	Endometrium	EEC	protein polyubiquitination	46/2168	236/18723	2.65e-04	3.04e-03	46
GO:003304413	Endometrium	EEC	regulation of chromosome organization	37/2168	187/18723	7.51e-04	7.05e-03	37
GO:004477213	Endometrium	EEC	mitotic cell cycle phase transition	71/2168	424/18723	8.68e-04	7.97e-03	71
GO:009006812	Endometrium	EEC	positive regulation of cell cycle process	44/2168	236/18723	9.39e-04	8.42e-03	44
GO:004593113	Endometrium	EEC	positive regulation of mitotic cell cycle	25/2168	121/18723	2.76e-03	1.97e-02	25
GO:19019893	Endometrium	EEC	positive regulation of cell cycle phase transition	23/2168	115/18723	6.04e-03	3.57e-02	23
GO:00073467	Endometrium	EEC	regulation of mitotic cell cycle	70/2168	457/18723	8.76e-03	4.74e-02	70
GO:0010498111	Esophagus	ESCC	proteasomal protein catabolic process	369/8552	490/18723	1.13e-41	1.80e-38	369
GO:0043161111	Esophagus	ESCC	proteasome-mediated ubiquitin-dependent protein catabolic process	312/8552	412/18723	3.53e-36	4.48e-33	312

Page: 1 2 3

Enriched KEGG pathways.

Pathway ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	qvalue	Count
hsa0412016	Endometrium	AEH	Ubiquitin mediated proteolysis	41/1197	142/8465	3.42e-06	4.27e-05	3.13e-05	41
hsa0516626	Endometrium	AEH	Human T-cell leukemia virus 1 infection	50/1197	222/8465	4.30e-04	3.11e-03	2.27e-03	50
hsa0412017	Endometrium	AEH	Ubiquitin mediated proteolysis	41/1197	142/8465	3.42e-06	4.27e-05	3.13e-05	41
hsa05166111	Endometrium	AEH	Human T-cell leukemia virus 1 infection	50/1197	222/8465	4.30e-04	3.11e-03	2.27e-03	50
hsa0516627	Endometrium	EEC	Human T-cell leukemia virus 1 infection	52/1237	222/8465	2.73e-04	2.36e-03	1.76e-03	52
hsa0516636	Endometrium	EEC	Human T-cell leukemia virus 1 infection	52/1237	222/8465	2.73e-04	2.36e-03	1.76e-03	52
hsa0412027	Esophagus	ESCC	Ubiquitin mediated proteolysis	122/4205	142/8465	6.53e-20	7.29e-18	3.74e-18	122
hsa0411023	Esophagus	ESCC	Cell cycle	126/4205	157/8465	1.34e-15	5.60e-14	2.87e-14	126
hsa05166211	Esophagus	ESCC	Human T-cell leukemia virus 1 infection	164/4205	222/8465	8.13e-14	2.09e-12	1.07e-12	164
hsa041146	Esophagus	ESCC	Oocyte meiosis	77/4205	131/8465	2.19e-02	4.80e-02	2.46e-02	77
hsa0412036	Esophagus	ESCC	Ubiquitin mediated proteolysis	122/4205	142/8465	6.53e-20	7.29e-18	3.74e-18	122
hsa0411033	Esophagus	ESCC	Cell cycle	126/4205	157/8465	1.34e-15	5.60e-14	2.87e-14	126
hsa05166310	Esophagus	ESCC	Human T-cell leukemia virus 1 infection	164/4205	222/8465	8.13e-14	2.09e-12	1.07e-12	164
hsa0411411	Esophagus	ESCC	Oocyte meiosis	77/4205	131/8465	2.19e-02	4.80e-02	2.46e-02	77

Page: 1

Top

Cell-cell communication analysis

Identification of potential cell-cell interactions between two cell types and their ligand-receptor pairs for different disease states

Ligand

Receptor

LRpair

Pathway

Tissue

Disease Stage

Page: 1

Top

Single-cell gene regulatory network inference analysis

Find out the significant the regulons (TFs) and the target genes of each regulon across cell types for different disease states

Cell Type

Tissue

Disease Stage

Target Gene

RSS

Regulon Activity

∗The dot plots of a searched regulon are shown for all cell subpopulations in each disease state of each tissue based on the regulon specific score inferred using pySCENIC and by calculating the average expression.

Page: 1

Top

Somatic mutation of malignant transformation related genes

Annotation of somatic variants for genes involved in malignant transformation

Hugo Symbol

Variant Class

Variant Classification

dbSNP RS

HGVSc

HGVSp

HGVSp Short

SWISSPROT

BIOTYPE

SIFT

PolyPhen

Tumor Sample Barcode

Tissue

Histology

Sex

Age

Stage

Therapy Types

Drugs

Outcome

ANAPC4

SNV

Missense_Mutation

c.1396N>G

p.Arg466Gly

p.R466G

Q9UJX5

protein_coding

deleterious(0.03)

benign(0.182)

TCGA-5L-AAT1-01

Breast

breast invasive carcinoma

Female

<65

III/IV

Hormone Therapy

letrozol

ANAPC4

SNV

Missense_Mutation

novel

c.86N>G

p.Ser29Trp

p.S29W

Q9UJX5

protein_coding

deleterious(0)

probably_damaging(0.986)

TCGA-A2-A3XZ-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

carboplatin

ANAPC4

SNV

Missense_Mutation

novel

c.500N>C

p.Leu167Pro

p.L167P

Q9UJX5

protein_coding

deleterious(0)

probably_damaging(0.998)

TCGA-HN-A2NL-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

cyclophosphamide

ANAPC4

insertion

Frame_Shift_Ins

novel

c.2406_2407insAACCAACTCAGGAATGAAAAGGGGAT

p.Leu803AsnfsTer14

p.L803Nfs*14

Q9UJX5

protein_coding

TCGA-AR-A0U0-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Unknown

ANAPC4

deletion

Frame_Shift_Del

c.438delN

p.Asn148ThrfsTer67

p.N148Tfs*67

Q9UJX5

protein_coding

TCGA-D8-A1XQ-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Unknown

ANAPC4

deletion

Frame_Shift_Del

novel

c.1044delN

p.Ile349Ter

p.I349*

Q9UJX5

protein_coding

TCGA-D8-A27V-01

Breast

breast invasive carcinoma

Female

<65

I/II

Hormone Therapy

tamoxiphen

ANAPC4

SNV

Missense_Mutation

c.616N>T

p.Leu206Phe

p.L206F

Q9UJX5

protein_coding

tolerated(0.32)

benign(0.03)

TCGA-C5-A901-01

Cervix

cervical & endocervical cancer

Female

<65

I/II

Unknown

ANAPC4

SNV

Missense_Mutation

rs573056698

c.1703G>A

p.Arg568His

p.R568H

Q9UJX5

protein_coding

tolerated(0.17)

benign(0)

TCGA-MA-AA42-01

Cervix

cervical & endocervical cancer

Female

>=65

I/II

Unknown

ANAPC4

SNV

Missense_Mutation

c.463N>C

p.Ile155Leu

p.I155L

Q9UJX5

protein_coding

deleterious(0.03)

probably_damaging(0.952)

TCGA-AA-A00N-01

Colorectum

colon adenocarcinoma

Male

>=65

I/II

Unknown

ANAPC4

SNV

Missense_Mutation

novel

c.133N>G

p.Leu45Val

p.L45V

Q9UJX5

protein_coding

tolerated(0.28)

probably_damaging(0.997)

TCGA-CA-6717-01

Colorectum

colon adenocarcinoma

Male

<65

I/II

Chemotherapy

oxaliplatin

Page: 1 2 3 4 5 6

Top

Related drugs of malignant transformation related genes

Identification of chemicals and drugs interact with genes involved in malignant transfromation

(DGIdb 4.0)

Entrez ID

Symbol

Category

Interaction Types

Drug Claim Name

Drug Name

PMIDs

Page: 1