Schematic overview of the cellular and molecular mechanisms involved in the cancer progression, including the proposed cellular and molecular mechanisms in cancer cells trajectory. AT1: alveolar type 1 cells; AT2: alveolar type 2 cells; AAH: atypical adenomatous hyperplasia; AIS: adenocarcinoma in situ; MIA: minimally invasive adenocarcinoma; IA: invasive adenocarcinoma; EMT: epithelial-mesenchymal transition

Home

Download

Statistics

Help

Contact

	Gene summary
	Malignant transformation analysis
	Malignant transformation related pathway analysis
	Cell-cell communication analysis
	Single-cell gene regulatory network inference analysis
	Somatic mutation of malignant transformation related genes
	Related drugs of malignant transformation related genes

Gene: MAP3K5

Gene summary for MAP3K5

Gene summary.

Gene information	Species	Human
	Gene symbol	MAP3K5
	Gene ID	4217
	Gene name	mitogen-activated protein kinase kinase kinase 5
	Gene Alias	ASK1
	Cytomap	6q23.3
	Gene Type	protein-coding
	GO ID	GO:0000165
	UniProtAcc	Q99683

Top

Malignant transformation analysis

Identification of the aberrant gene expression in precancerous and cancerous lesions by comparing the gene expression of stem-like cells in diseased tissues with normal stem cells

Malignant transformation involving gene list.

Entrez ID	Symbol	Replicates	Species	Organ	Tissue	Adj P-value	Log2FC	Malignancy
4217	MAP3K5	CCI_1	Human	Cervix	CC	2.13e-11	1.20e+00	0.528
4217	MAP3K5	CCI_2	Human	Cervix	CC	2.44e-05	1.23e+00	0.5249
4217	MAP3K5	CCI_3	Human	Cervix	CC	2.65e-07	7.74e-01	0.516
4217	MAP3K5	HTA11_3410_2000001011	Human	Colorectum	AD	1.38e-02	-3.23e-01	0.0155
4217	MAP3K5	HTA11_347_2000001011	Human	Colorectum	AD	4.39e-10	6.12e-01	-0.1954
4217	MAP3K5	HTA11_866_3004761011	Human	Colorectum	AD	4.51e-02	-4.11e-01	0.096
4217	MAP3K5	HTA11_99999974143_84620	Human	Colorectum	MSS	3.97e-19	-5.86e-01	0.3005
4217	MAP3K5	F007	Human	Colorectum	FAP	5.33e-03	-3.60e-01	0.1176
4217	MAP3K5	A015-C-203	Human	Colorectum	FAP	1.63e-22	-5.09e-01	-0.1294
4217	MAP3K5	A015-C-204	Human	Colorectum	FAP	4.03e-04	-3.22e-01	-0.0228
4217	MAP3K5	A014-C-040	Human	Colorectum	FAP	3.06e-02	-4.22e-01	-0.1184
4217	MAP3K5	A002-C-201	Human	Colorectum	FAP	8.18e-08	-2.98e-01	0.0324
4217	MAP3K5	A001-C-119	Human	Colorectum	FAP	3.80e-08	-5.06e-01	-0.1557
4217	MAP3K5	A001-C-108	Human	Colorectum	FAP	9.73e-11	-2.13e-01	-0.0272
4217	MAP3K5	A002-C-205	Human	Colorectum	FAP	4.08e-19	-5.00e-01	-0.1236
4217	MAP3K5	A015-C-005	Human	Colorectum	FAP	3.23e-04	-4.07e-01	-0.0336
4217	MAP3K5	A015-C-006	Human	Colorectum	FAP	1.37e-13	-5.06e-01	-0.0994
4217	MAP3K5	A015-C-106	Human	Colorectum	FAP	2.27e-09	-2.73e-01	-0.0511
4217	MAP3K5	A002-C-114	Human	Colorectum	FAP	1.20e-16	-5.11e-01	-0.1561
4217	MAP3K5	A015-C-104	Human	Colorectum	FAP	8.54e-24	-4.96e-01	-0.1899

Page: 1 2 3 4 5 6

Transcriptomic changes along malignancy continuum.

Tissue	Expression Dynamics	Abbreviation
Cervix		CC: Cervix cancer
		HSIL_HPV: HPV-infected high-grade squamous intraepithelial lesions
		N_HPV: HPV-infected normal cervix
Colorectum (GSE201348)		FAP: Familial adenomatous polyposis
		CRC: Colorectal cancer
Colorectum (HTA11)		AD: Adenomas
		SER: Sessile serrated lesions
		MSI-H: Microsatellite-high colorectal cancer
		MSS: Microsatellite stable colorectal cancer
Endometrium		AEH: Atypical endometrial hyperplasia
		EEC: Endometrioid Cancer
Liver		HCC: Hepatocellular carcinoma
		NAFLD: Non-alcoholic fatty liver disease
Lung		AAH: Atypical adenomatous hyperplasia
		AIS: Adenocarcinoma in situ
		IAC: Invasive lung adenocarcinoma
		MIA: Minimally invasive adenocarcinoma
Oral Cavity		EOLP: Erosive Oral lichen planus
		LP: leukoplakia
		NEOLP: Non-erosive oral lichen planus
		OSCC: Oral squamous cell carcinoma
Prostate		BPH: Benign Prostatic Hyperplasia
Thyroid		ATC: Anaplastic thyroid cancer
		HT: Hashimoto's thyroiditis
		PTC: Papillary thyroid cancer

∗log2FC in expression of this searched gene in stem-like cells from each diseased tissue sample relative to stem-like cells in normal samples in each tissue plotted against the malignancy continuum. Samples are colored based on if they are from different disease stage.

Top

Malignant transformation related pathway analysis

Find out the enriched GO biological processes and KEGG pathways involved in transition from healthy to precancer to cancer

Figure of enriched GO biological processes.

Tissue	Disease Stage	Enriched GO biological Processes
Colorectum	AD
Colorectum	SER
Colorectum	MSS
Colorectum	MSI-H
Colorectum	FAP

∗Top 15 enriched GO BP terms are showed in the bar plot of each disease state in each tissue. Each row represents a significant GO biological process which is colored according to the -log10(p.adjust).

Page: 1 2 3 4 5 6 7 8 9

Enriched GO biological processes.

GO ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	Count
GO:009719310	Cervix	CC	intrinsic apoptotic signaling pathway	85/2311	288/18723	4.56e-15	6.46e-12	85
GO:004206010	Cervix	CC	wound healing	109/2311	422/18723	1.84e-14	1.57e-11	109
GO:005254710	Cervix	CC	regulation of peptidase activity	112/2311	461/18723	6.70e-13	3.08e-10	112
GO:004586210	Cervix	CC	positive regulation of proteolysis	95/2311	372/18723	1.84e-12	6.86e-10	95
GO:005254810	Cervix	CC	regulation of endopeptidase activity	103/2311	432/18723	1.89e-11	5.14e-09	103
GO:000697910	Cervix	CC	response to oxidative stress	102/2311	446/18723	2.99e-10	4.36e-08	102
GO:007149610	Cervix	CC	cellular response to external stimulus	78/2311	320/18723	1.82e-09	1.98e-07	78
GO:001095210	Cervix	CC	positive regulation of peptidase activity	54/2311	197/18723	8.06e-09	6.99e-07	54
GO:003367410	Cervix	CC	positive regulation of kinase activity	101/2311	467/18723	8.66e-09	7.22e-07	101
GO:200011610	Cervix	CC	regulation of cysteine-type endopeptidase activity	61/2311	235/18723	8.69e-09	7.22e-07	61
GO:004328110	Cervix	CC	regulation of cysteine-type endopeptidase activity involved in apoptotic process	55/2311	209/18723	2.77e-08	1.95e-06	55
GO:00458608	Cervix	CC	positive regulation of protein kinase activity	85/2311	386/18723	5.61e-08	3.29e-06	85
GO:000030210	Cervix	CC	response to reactive oxygen species	56/2311	222/18723	1.00e-07	5.25e-06	56
GO:006219710	Cervix	CC	cellular response to chemical stress	76/2311	337/18723	1.01e-07	5.25e-06	76
GO:001095010	Cervix	CC	positive regulation of endopeptidase activity	48/2311	179/18723	1.12e-07	5.71e-06	48
GO:00075689	Cervix	CC	aging	75/2311	339/18723	2.75e-07	1.13e-05	75
GO:00316689	Cervix	CC	cellular response to extracellular stimulus	59/2311	246/18723	3.03e-07	1.24e-05	59
GO:004254210	Cervix	CC	response to hydrogen peroxide	40/2311	146/18723	6.79e-07	2.46e-05	40
GO:00310988	Cervix	CC	stress-activated protein kinase signaling cascade	58/2311	247/18723	8.02e-07	2.89e-05	58
GO:00719007	Cervix	CC	regulation of protein serine/threonine kinase activity	75/2311	359/18723	2.76e-06	8.02e-05	75

Page: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46

Enriched KEGG pathways.

Pathway ID	Tissue	Disease Stage	Description	Gene Ratio	Bg Ratio	pvalue	p.adjust	qvalue	Count
hsa0501226	Endometrium	AEH	Parkinson disease	115/1197	266/8465	6.24e-32	1.01e-29	7.42e-30	115
hsa0501620	Endometrium	AEH	Huntington disease	118/1197	306/8465	3.00e-27	2.44e-25	1.78e-25	118
hsa0520826	Endometrium	AEH	Chemical carcinogenesis - reactive oxygen species	91/1197	223/8465	3.28e-23	1.33e-21	9.76e-22	91
hsa0501420	Endometrium	AEH	Amyotrophic lateral sclerosis	123/1197	364/8465	2.17e-22	7.82e-21	5.72e-21	123
hsa0501020	Endometrium	AEH	Alzheimer disease	124/1197	384/8465	1.20e-20	3.91e-19	2.86e-19	124
hsa0493220	Endometrium	AEH	Non-alcoholic fatty liver disease	67/1197	155/8465	5.91e-19	1.75e-17	1.28e-17	67
hsa0471426	Endometrium	AEH	Thermogenesis	85/1197	232/8465	3.44e-18	9.33e-17	6.83e-17	85
hsa0502220	Endometrium	AEH	Pathways of neurodegeneration - multiple diseases	137/1197	476/8465	9.27e-18	2.32e-16	1.70e-16	137
hsa0414126	Endometrium	AEH	Protein processing in endoplasmic reticulum	62/1197	174/8465	5.97e-13	1.39e-11	1.01e-11	62
hsa0453026	Endometrium	AEH	Tight junction	54/1197	169/8465	1.99e-09	3.80e-08	2.78e-08	54
hsa0541826	Endometrium	AEH	Fluid shear stress and atherosclerosis	43/1197	139/8465	2.47e-07	3.49e-06	2.55e-06	43
hsa0501718	Endometrium	AEH	Spinocerebellar ataxia	43/1197	143/8465	5.92e-07	7.69e-06	5.63e-06	43
hsa0472216	Endometrium	AEH	Neurotrophin signaling pathway	31/1197	119/8465	4.07e-04	3.07e-03	2.25e-03	31
hsa0541726	Endometrium	AEH	Lipid and atherosclerosis	47/1197	215/8465	1.24e-03	7.62e-03	5.58e-03	47
hsa0421010	Endometrium	AEH	Apoptosis	30/1197	136/8465	7.72e-03	4.05e-02	2.96e-02	30
hsa05012111	Endometrium	AEH	Parkinson disease	115/1197	266/8465	6.24e-32	1.01e-29	7.42e-30	115
hsa05016110	Endometrium	AEH	Huntington disease	118/1197	306/8465	3.00e-27	2.44e-25	1.78e-25	118
hsa05208112	Endometrium	AEH	Chemical carcinogenesis - reactive oxygen species	91/1197	223/8465	3.28e-23	1.33e-21	9.76e-22	91
hsa05014110	Endometrium	AEH	Amyotrophic lateral sclerosis	123/1197	364/8465	2.17e-22	7.82e-21	5.72e-21	123
hsa05010110	Endometrium	AEH	Alzheimer disease	124/1197	384/8465	1.20e-20	3.91e-19	2.86e-19	124

Page: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Top

Cell-cell communication analysis

Identification of potential cell-cell interactions between two cell types and their ligand-receptor pairs for different disease states

Ligand

Receptor

LRpair

Pathway

Tissue

Disease Stage

Page: 1

Top

Single-cell gene regulatory network inference analysis

Find out the significant the regulons (TFs) and the target genes of each regulon across cell types for different disease states

Cell Type

Tissue

Disease Stage

Target Gene

RSS

Regulon Activity

∗The dot plots of a searched regulon are shown for all cell subpopulations in each disease state of each tissue based on the regulon specific score inferred using pySCENIC and by calculating the average expression.

Page: 1

Top

Somatic mutation of malignant transformation related genes

Annotation of somatic variants for genes involved in malignant transformation

Hugo Symbol

Variant Class

Variant Classification

dbSNP RS

HGVSc

HGVSp

HGVSp Short

SWISSPROT

BIOTYPE

SIFT

PolyPhen

Tumor Sample Barcode

Tissue

Histology

Sex

Age

Stage

Therapy Types

Drugs

Outcome

MAP3K5

SNV

Missense_Mutation

rs771379166

c.3427C>T

p.Arg1143Trp

p.R1143W

Q99683

protein_coding

deleterious(0)

probably_damaging(0.993)

TCGA-AC-A23H-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Unknown

MAP3K5

SNV

Missense_Mutation

c.1276N>A

p.Glu426Lys

p.E426K

Q99683

protein_coding

deleterious(0)

probably_damaging(0.995)

TCGA-AC-A2B8-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Chemotherapy

chemo

MAP3K5

SNV

Missense_Mutation

c.3040C>T

p.Arg1014Trp

p.R1014W

Q99683

protein_coding

deleterious(0.02)

probably_damaging(0.993)

TCGA-AN-A046-01

Breast

breast invasive carcinoma

Female

>=65

I/II

Unknown

MAP3K5

SNV

Missense_Mutation

c.702N>C

p.Leu234Phe

p.L234F

Q99683

protein_coding

tolerated(0.08)

possibly_damaging(0.883)

TCGA-AN-A0AR-01

Breast

breast invasive carcinoma

Female

<65

I/II

Unknown

MAP3K5

SNV

Missense_Mutation

novel

c.1307N>A

p.Ala436Glu

p.A436E

Q99683

protein_coding

deleterious(0)

probably_damaging(0.999)

TCGA-AN-A0FZ-01

Breast

breast invasive carcinoma

Female

<65

III/IV

Unknown

MAP3K5

SNV

Missense_Mutation

novel

c.1531N>T

p.Leu511Phe

p.L511F

Q99683

protein_coding

deleterious(0)

probably_damaging(0.998)

TCGA-BH-A0B7-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

adriamycin

MAP3K5

SNV

Missense_Mutation

novel

c.2311N>A

p.Gly771Ser

p.G771S

Q99683

protein_coding

deleterious(0)

possibly_damaging(0.896)

TCGA-BH-A0BL-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

adriamycin

MAP3K5

SNV

Missense_Mutation

c.1276G>A

p.Glu426Lys

p.E426K

Q99683

protein_coding

deleterious(0)

probably_damaging(0.995)

TCGA-BH-A0DZ-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

docetaxel

MAP3K5

SNV

Missense_Mutation

novel

c.3817G>A

p.Ala1273Thr

p.A1273T

Q99683

protein_coding

tolerated(0.79)

benign(0.257)

TCGA-E2-A14R-01

Breast

breast invasive carcinoma

Female

<65

I/II

Chemotherapy

doxorubicin

MAP3K5

SNV

Missense_Mutation

novel

c.850C>G

p.Arg284Gly

p.R284G

Q99683

protein_coding

deleterious(0)

probably_damaging(0.997)

TCGA-GI-A2C9-01

Breast

breast invasive carcinoma

Female

<65

I/II

Unspecific

Page: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Top

Related drugs of malignant transformation related genes

Identification of chemicals and drugs interact with genes involved in malignant transfromation

(DGIdb 4.0)

Entrez ID	Symbol	Category	Interaction Types	Drug Claim Name	Drug Name	PMIDs
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME		GS-4997	SELONSERTIB
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	249565756
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME		GS-4977
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	249565813
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	385612190
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	CHEMBL3545429	SELONSERTIB
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME		hydroxyurea	HYDROXYUREA	23556445
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	315661125	SELONSERTIB
4217	MAP3K5	SERINE THREONINE KINASE, KINASE, DRUGGABLE GENOME, EXTERNAL SIDE OF PLASMA MEMBRANE, TRANSCRIPTION FACTOR, ENZYME	inhibitor	404859049

Page: 1