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Gene: WASHC5 |
Gene summary for WASHC5 |
 Gene summary. |
| Gene information | Species | Human | Gene symbol | WASHC5 | Gene ID | 9897 |
| Gene name | WASH complex subunit 5 | |
| Gene Alias | KIAA0196 | |
| Cytomap | 8q24.13 | |
| Gene Type | protein-coding | GO ID | GO:0000003 | UniProtAcc | E7EQI7 |
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Malignant transformation analysis |
| Identification of the aberrant gene expression in precancerous and cancerous lesions by comparing the gene expression of stem-like cells in diseased tissues with normal stem cells |
| Malignant transformation involving gene list. |
| Entrez ID | Symbol | Replicates | Species | Organ | Tissue | Adj P-value | Log2FC | Malignancy |
| 9897 | WASHC5 | HCC1 | Human | Liver | HCC | 7.45e-06 | 2.75e+00 | 0.5336 |
| 9897 | WASHC5 | HCC2 | Human | Liver | HCC | 3.03e-22 | 3.84e+00 | 0.5341 |
| 9897 | WASHC5 | S014 | Human | Liver | HCC | 1.04e-33 | 8.62e-01 | 0.2254 |
| 9897 | WASHC5 | S015 | Human | Liver | HCC | 3.39e-30 | 9.39e-01 | 0.2375 |
| 9897 | WASHC5 | S016 | Human | Liver | HCC | 6.57e-31 | 8.17e-01 | 0.2243 |
| 9897 | WASHC5 | S027 | Human | Liver | HCC | 4.42e-21 | 1.19e+00 | 0.2446 |
| 9897 | WASHC5 | S028 | Human | Liver | HCC | 7.73e-41 | 1.37e+00 | 0.2503 |
| 9897 | WASHC5 | S029 | Human | Liver | HCC | 7.12e-45 | 1.64e+00 | 0.2581 |
| Page: 1 |
| Transcriptomic changes along malignancy continuum. |
| Tissue | Expression Dynamics | Abbreviation |
| Liver |  | HCC: Hepatocellular carcinoma |
| NAFLD: Non-alcoholic fatty liver disease |
| ∗log2FC in expression of this searched gene in stem-like cells from each diseased tissue sample relative to stem-like cells in normal samples in each tissue plotted against the malignancy continuum. Samples are colored based on if they are from different disease stage. |
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Malignant transformation related pathway analysis |
| Find out the enriched GO biological processes and KEGG pathways involved in transition from healthy to precancer to cancer |
| Figure of enriched GO biological processes. |
| Tissue | Disease Stage | Enriched GO biological Processes |
| Colorectum | AD |  |
| Colorectum | SER |  |
| Colorectum | MSS |  |
| Colorectum | MSI-H |  |
| Colorectum | FAP |  |
| ∗Top 15 enriched GO BP terms are showed in the bar plot of each disease state in each tissue. Each row represents a significant GO biological process which is colored according to the -log10(p.adjust). |
| Page: 1 2 3 4 5 6 7 8 9 |
| Enriched GO biological processes. |
| GO ID | Tissue | Disease Stage | Description | Gene Ratio | Bg Ratio | pvalue | p.adjust | Count |
| GO:00315031 | Liver | HCC | protein-containing complex localization | 129/7958 | 220/18723 | 9.38e-07 | 1.42e-05 | 129 |
| GO:003083212 | Liver | HCC | regulation of actin filament length | 113/7958 | 189/18723 | 1.16e-06 | 1.71e-05 | 113 |
| GO:003083322 | Liver | HCC | regulation of actin filament polymerization | 104/7958 | 172/18723 | 1.46e-06 | 2.08e-05 | 104 |
| GO:000806412 | Liver | HCC | regulation of actin polymerization or depolymerization | 112/7958 | 188/18723 | 1.65e-06 | 2.31e-05 | 112 |
| GO:005122511 | Liver | HCC | spindle assembly | 75/7958 | 117/18723 | 1.92e-06 | 2.67e-05 | 75 |
| GO:003134611 | Liver | HCC | positive regulation of cell projection organization | 193/7958 | 353/18723 | 2.28e-06 | 3.08e-05 | 193 |
| GO:0048285 | Liver | HCC | organelle fission | 254/7958 | 488/18723 | 1.07e-05 | 1.23e-04 | 254 |
| GO:003083812 | Liver | HCC | positive regulation of actin filament polymerization | 63/7958 | 99/18723 | 1.76e-05 | 1.89e-04 | 63 |
| GO:000091011 | Liver | HCC | cytokinesis | 100/7958 | 173/18723 | 3.38e-05 | 3.39e-04 | 100 |
| GO:00070401 | Liver | HCC | lysosome organization | 47/7958 | 74/18723 | 2.13e-04 | 1.63e-03 | 47 |
| GO:00801711 | Liver | HCC | lytic vacuole organization | 47/7958 | 74/18723 | 2.13e-04 | 1.63e-03 | 47 |
| GO:004501011 | Liver | HCC | actin nucleation | 35/7958 | 52/18723 | 2.63e-04 | 1.94e-03 | 35 |
| GO:006164011 | Liver | HCC | cytoskeleton-dependent cytokinesis | 60/7958 | 100/18723 | 3.09e-04 | 2.20e-03 | 60 |
| GO:0000280 | Liver | HCC | nuclear division | 220/7958 | 439/18723 | 6.90e-04 | 4.25e-03 | 220 |
| GO:0034629 | Liver | HCC | cellular protein-containing complex localization | 17/7958 | 22/18723 | 9.84e-04 | 5.70e-03 | 17 |
| GO:001097511 | Liver | HCC | regulation of neuron projection development | 220/7958 | 445/18723 | 1.68e-03 | 8.78e-03 | 220 |
| GO:0010976 | Liver | HCC | positive regulation of neuron projection development | 88/7958 | 163/18723 | 1.98e-03 | 1.00e-02 | 88 |
| GO:00511254 | Liver | HCC | regulation of actin nucleation | 21/7958 | 30/18723 | 2.15e-03 | 1.08e-02 | 21 |
| GO:0097494 | Liver | HCC | regulation of vesicle size | 9/7958 | 11/18723 | 9.51e-03 | 3.60e-02 | 9 |
| Page: 1 2 |
| Enriched KEGG pathways. |
| Pathway ID | Tissue | Disease Stage | Description | Gene Ratio | Bg Ratio | pvalue | p.adjust | qvalue | Count |
| hsa0414422 | Liver | HCC | Endocytosis | 178/4020 | 251/8465 | 2.03e-14 | 5.22e-13 | 2.91e-13 | 178 |
| hsa0414432 | Liver | HCC | Endocytosis | 178/4020 | 251/8465 | 2.03e-14 | 5.22e-13 | 2.91e-13 | 178 |
| Page: 1 |
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Cell-cell communication analysis |
| Identification of potential cell-cell interactions between two cell types and their ligand-receptor pairs for different disease states |
| Ligand | Receptor | LRpair | Pathway | Tissue | Disease Stage |
| Page: 1 |
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Single-cell gene regulatory network inference analysis |
| Find out the significant the regulons (TFs) and the target genes of each regulon across cell types for different disease states |
| TF | Cell Type | Tissue | Disease Stage | Target Gene | RSS | Regulon Activity |
| ∗The dot plots of a searched regulon are shown for all cell subpopulations in each disease state of each tissue based on the regulon specific score inferred using pySCENIC and by calculating the average expression. |
| Page: 1 |
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Somatic mutation of malignant transformation related genes |
| Annotation of somatic variants for genes involved in malignant transformation |
| Hugo Symbol | Variant Class | Variant Classification | dbSNP RS | HGVSc | HGVSp | HGVSp Short | SWISSPROT | BIOTYPE | SIFT | PolyPhen | Tumor Sample Barcode | Tissue | Histology | Sex | Age | Stage | Therapy Types | Drugs | Outcome |
| WASHC5 | SNV | Missense_Mutation | novel | c.343N>A | p.Asp115Asn | p.D115N | Q12768 | protein_coding | deleterious(0) | benign(0.259) | TCGA-A8-A09D-01 | Breast | breast invasive carcinoma | Female | <65 | I/II | Chemotherapy | doxorubicin | CR |
| WASHC5 | SNV | Missense_Mutation | c.2862N>A | p.Met954Ile | p.M954I | Q12768 | protein_coding | tolerated(0.52) | benign(0) | TCGA-AC-A23H-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Unknown | Unknown | PD | |
| WASHC5 | SNV | Missense_Mutation | rs886062654 | c.1340N>T | p.Ser447Leu | p.S447L | Q12768 | protein_coding | tolerated(0.68) | possibly_damaging(0.473) | TCGA-AN-A046-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Unknown | Unknown | SD |
| WASHC5 | SNV | Missense_Mutation | novel | c.1850N>A | p.Gly617Glu | p.G617E | Q12768 | protein_coding | tolerated(0.16) | probably_damaging(0.999) | TCGA-BH-A0HF-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Hormone Therapy | arimidex | SD |
| WASHC5 | SNV | Missense_Mutation | rs746215387 | c.136N>A | p.Ala46Thr | p.A46T | Q12768 | protein_coding | tolerated(0.5) | benign(0.005) | TCGA-BH-A0HF-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Hormone Therapy | arimidex | SD |
| WASHC5 | SNV | Missense_Mutation | novel | c.2897N>G | p.Asn966Ser | p.N966S | Q12768 | protein_coding | tolerated(0.27) | benign(0.018) | TCGA-LD-A7W5-01 | Breast | breast invasive carcinoma | Female | <65 | III/IV | Chemotherapy | taxotere | SD |
| WASHC5 | insertion | Nonsense_Mutation | novel | c.1091_1092insTGGTTACTAGTATATTAGATGTTTCTTT | p.Cys365GlyfsTer3 | p.C365Gfs*3 | Q12768 | protein_coding | TCGA-A8-A06X-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Unknown | Unknown | SD | ||
| WASHC5 | deletion | Frame_Shift_Del | c.2174delG | p.Gly725AspfsTer2 | p.G725Dfs*2 | Q12768 | protein_coding | TCGA-AN-A0AK-01 | Breast | breast invasive carcinoma | Female | >=65 | I/II | Unknown | Unknown | SD | |||
| WASHC5 | SNV | Missense_Mutation | novel | c.2892N>T | p.Glu964Asp | p.E964D | Q12768 | protein_coding | deleterious(0.02) | probably_damaging(0.982) | TCGA-2W-A8YY-01 | Cervix | cervical & endocervical cancer | Female | <65 | I/II | Chemotherapy | cisplatin | CR |
| WASHC5 | SNV | Missense_Mutation | rs772770553 | c.2488N>T | p.Arg830Trp | p.R830W | Q12768 | protein_coding | deleterious(0) | probably_damaging(0.995) | TCGA-2W-A8YY-01 | Cervix | cervical & endocervical cancer | Female | <65 | I/II | Chemotherapy | cisplatin | CR |
| Page: 1 2 3 4 5 6 7 8 9 10 11 12 |
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Related drugs of malignant transformation related genes |
| Identification of chemicals and drugs interact with genes involved in malignant transfromation |
| (DGIdb 4.0) |
| Entrez ID | Symbol | Category | Interaction Types | Drug Claim Name | Drug Name | PMIDs |
| Page: 1 |
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