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Fusion gene ID: 12093

FusionGeneSummary for ESR1_MTHFD1L

Fusion gene summary

Fusion gene information	Fusion gene name: ESR1_MTHFD1L
	Fusion gene ID: 12093
		Hgene	Tgene
	Gene symbol	ESR1	MTHFD1L
	Gene ID	2099	25902
	Gene name	estrogen receptor 1	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
	Synonyms	ER\|ESR\|ESRA\|ESTRR\|Era\|NR3A1	FTHFSDC1\|MTC1THFS\|dJ292B18.2
	Cytomap	6q25.1-q25.2	6q25.1
	Type of gene	protein-coding	protein-coding
	Description	estrogen receptorE2 receptor alphaER-alphaestradiol receptorestrogen nuclear receptor alphaestrogen receptor alpha E1-E2-1-2estrogen receptor alpha E1-N2-E2-1-2nuclear receptor subfamily 3 group A member 1oestrogen receptor alpha	monofunctional C1-tetrahydrofolate synthase, mitochondrial10-formyl-THF synthetaseformyltetrahydrofolate synthetase domain containing 1
	Modification date	20180527	20180523
	UniProtAcc	P03372	Q6UB35
	Ensembl transtripts involved in fusion gene	ENST00000440973, ENST00000338799, ENST00000456483, ENST00000443427, ENST00000206249, ENST00000406599, ENST00000427531, ENST00000482101,	ENST00000367321, ENST00000367307, ENST00000478643,
Fusion gene scores	* DoF score	12 X 7 X 4=336	9 X 8 X 4=288
	# samples	12	14
	** MAII score	log2(12/336*10)=-1.48542682717024 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(14/288*10)=-1.04064198449735 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: ESR1 [Title/Abstract] AND MTHFD1L [Title/Abstract] AND fusion [Title/Abstract]
Functional or gene categories assigned by FusionGDB annotation	Oncogene involved fusion gene, in-frame and retained their domain. Tumor suppressor gene involved fusion gene, in-frame but not retained their domain. Transcription factor involved fusion gene, inframe and retained DNA-binding domain.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ESR1	GO:0006366	transcription by RNA polymerase II	15831516
Hgene	ESR1	GO:0010629	negative regulation of gene expression	21695196
Hgene	ESR1	GO:0030520	intracellular estrogen receptor signaling pathway	9841876
Hgene	ESR1	GO:0032355	response to estradiol	15304487
Hgene	ESR1	GO:0043124	negative regulation of I-kappaB kinase/NF-kappaB signaling	7651415\|16043358
Hgene	ESR1	GO:0043433	negative regulation of DNA binding transcription factor activity	10816575
Hgene	ESR1	GO:0043627	response to estrogen	11581164
Hgene	ESR1	GO:0045893	positive regulation of transcription, DNA-templated	9841876\|20074560
Hgene	ESR1	GO:0045899	positive regulation of RNA polymerase II transcriptional preinitiation complex assembly	9841876
Hgene	ESR1	GO:0045944	positive regulation of transcription by RNA polymerase II	11544182\|12047722\|15345745\|15831516\|18563714
Hgene	ESR1	GO:0051091	positive regulation of DNA binding transcription factor activity	9328340\|10681512
Hgene	ESR1	GO:0071392	cellular response to estradiol stimulus	15831516
Tgene	MTHFD1L	GO:0006760	folic acid-containing compound metabolic process	12937168
Tgene	MTHFD1L	GO:0015942	formate metabolic process	16171773
Tgene	MTHFD1L	GO:0046653	tetrahydrofolate metabolic process	12937168

Fusion gene information from three resources
(ChiTars (NAR, 2018), tumorfusions (NAR, 2018), Gao et al. (Cell, 2018))
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Data type	Source	Cancer type	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
TCGA	RV	BRCA	TCGA-BH-A5J0-01A	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+

* LD: Li Ding group's fusion gene list
RV: Roel Verhaak group's fusion gene list
ChiTaRs fusion database

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
In-frame	ENST00000440973	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-intron	ENST00000440973	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-3UTR	ENST00000440973	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
In-frame	ENST00000338799	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-intron	ENST00000338799	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-3UTR	ENST00000338799	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-3CDS	ENST00000456483	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-intron	ENST00000456483	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-3UTR	ENST00000456483	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
In-frame	ENST00000443427	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-intron	ENST00000443427	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-3UTR	ENST00000443427	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
In-frame	ENST00000206249	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-intron	ENST00000206249	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-3UTR	ENST00000206249	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-3CDS	ENST00000406599	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-intron	ENST00000406599	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
intron-3UTR	ENST00000406599	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
In-frame	ENST00000427531	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-intron	ENST00000427531	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
5CDS-3UTR	ENST00000427531	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
3UTR-3CDS	ENST00000482101	ENST00000367321	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
3UTR-intron	ENST00000482101	ENST00000367307	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+
3UTR-3UTR	ENST00000482101	ENST00000478643	ESR1	chr6	152265643	+	MTHFD1L	chr6	151330955	+

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FusionProtFeatures for ESR1_MTHFD1L

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ESR1 P03372	MTHFD1L Q6UB35
Nuclear hormone receptor. The steroid hormones and theirreceptors are involved in the regulation of eukaryotic geneexpression and affect cellular proliferation and differentiationin target tissues. Ligand-dependent nuclear transactivationinvolves either direct homodimer binding to a palindromic estrogenresponse element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2,Sp1 and Sp3, to mediate ERE-independent signaling. Ligand bindinginduces a conformational change allowing subsequent orcombinatorial association with multiprotein coactivator complexesthrough LXXLL motifs of their respective components. Mutualtransrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcriptionfrom the IL6 promoter and displace RELA/p65 and associatedcoregulators from the promoter. Recruited to the NF-kappa-Bresponse element of the CCL2 and IL8 promoters and can displaceCREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50on ERE sequences. Can also act synergistically with NF-kappa-B toactivate transcription involving respective recruitment adjacentresponse elements; the function involves CREBBP. Can activate thetranscriptional activity of TFF1. Also mediates membrane-initiatedestrogen signaling involving various kinase cascades. Isoform 3 isinvolved in activation of NOS3 and endothelial nitric oxideproduction. Isoforms lacking one or several functional domains arethought to modulate transcriptional activity by competitive ligandor DNA binding and/or heterodimerization with the full-lengthreceptor. Essential for MTA1-mediated transcriptional regulationof BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform1. {ECO:0000269\|PubMed:10681512, ECO:0000269\|PubMed:10816575,ECO:0000269\|PubMed:11477071, ECO:0000269\|PubMed:11682626,ECO:0000269\|PubMed:14764652, ECO:0000269\|PubMed:15078875,ECO:0000269\|PubMed:15891768, ECO:0000269\|PubMed:16043358,ECO:0000269\|PubMed:16617102, ECO:0000269\|PubMed:16684779,ECO:0000269\|PubMed:17922032, ECO:0000269\|PubMed:17932106,ECO:0000269\|PubMed:18247370, ECO:0000269\|PubMed:19350539,ECO:0000269\|PubMed:20074560, ECO:0000269\|PubMed:20705611,ECO:0000269\|PubMed:21330404, ECO:0000269\|PubMed:22083956,ECO:0000269\|PubMed:7651415, ECO:0000269\|PubMed:9328340}.	May provide the missing metabolic reaction required tolink the mitochondria and the cytoplasm in the mammalian model ofone-carbon folate metabolism in embryonic an transformed cellscomplementing thus the enzymatic activities of MTHFD2.{ECO:0000250, ECO:0000269\|PubMed:16171773}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
.

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

>>>>

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

185_250

365

596

DNA binding

Nuclear receptor

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

185_250

365

596

DNA binding

Nuclear receptor

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

185_250

365

596

DNA binding

Nuclear receptor

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

185_250

365

596

DNA binding

Nuclear receptor

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

251_310

365

596

Region

Note=Hinge

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

251_310

365

596

Region

Note=Hinge

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

251_310

365

596

Region

Note=Hinge

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

251_310

365

596

Region

Note=Hinge

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

185_205

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

221_245

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

185_205

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

221_245

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

185_205

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

221_245

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

185_205

365

596

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

221_245

365

596

Zinc finger

NR C4-type

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367307

34_42

-90

276

Compositional bias

Note=Poly-Gly

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367307

423_430

-90

276

Nucleotide binding

ATP

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367307

31_348

-90

276

Region

Note=Methylenetetrahydrofolate dehydrogenase and cyclohydrolase

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367307

349_978

-90

276

Region

Note=Formyltetrahydrofolate synthetase

- In-frame and not-retained protein feature among the 13 regional features.

>>>>>>>>>>>>>>>>>>>

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

185_250

-73

484

DNA binding

Nuclear receptor

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

311_547

365

596

Domain

NR LBD

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

311_547

365

596

Domain

NR LBD

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

311_547

365

596

Domain

NR LBD

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

311_547

365

596

Domain

NR LBD

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

311_547

-73

484

Domain

NR LBD

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

264_595

365

596

Region

Note=Self-association

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000206249

311_595

365

596

Region

Note=Transactivation AF-2

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

264_595

365

596

Region

Note=Self-association

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000338799

311_595

365

596

Region

Note=Transactivation AF-2

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

264_595

365

596

Region

Note=Self-association

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000440973

311_595

365

596

Region

Note=Transactivation AF-2

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

264_595

365

596

Region

Note=Self-association

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000443427

311_595

365

596

Region

Note=Transactivation AF-2

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

251_310

-73

484

Region

Note=Hinge

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

264_595

-73

484

Region

Note=Self-association

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

311_595

-73

484

Region

Note=Transactivation AF-2

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

185_205

-73

484

Zinc finger

NR C4-type

Hgene

ESR1

chr6:152265643

chr6:151330955

ENST00000456483

221_245

-73

484

Zinc finger

NR C4-type

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367321

34_42

708

932

Compositional bias

Note=Poly-Gly

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367321

423_430

708

932

Nucleotide binding

ATP

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367321

31_348

708

932

Region

Note=Methylenetetrahydrofolate dehydrogenase and cyclohydrolase

Tgene

MTHFD1L

chr6:152265643

chr6:151330955

ENST00000367321

349_978

708

932

Region

Note=Formyltetrahydrofolate synthetase

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FusionGeneSequence for ESR1_MTHFD1L

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences.
(nt: nucleotides, aa: amino acids)

* Fusion amino acid sequences.

>In-frame_ESR1_ENST00000440973_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_636aa
MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFG
SNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK
ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQ
RDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW
AKRVPVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGV
PVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIE
LSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQ

>In-frame_ESR1_ENST00000338799_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_636aa
MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFG
SNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK
ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQ
RDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW
AKRVPVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGV
PVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIE
LSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQ

>In-frame_ESR1_ENST00000443427_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_636aa
MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFG
SNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK
ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQ
RDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW
AKRVPVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGV
PVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIE
LSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQ

>In-frame_ESR1_ENST00000206249_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_636aa
MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFG
SNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK
ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQ
RDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW
AKRVPVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGV
PVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIE
LSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQ

>In-frame_ESR1_ENST00000427531_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_463aa
MAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGR
MLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRE
LVHMINWAKRVPVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQ
ITQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAV
YGAKDIELSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDID

* Fusion transcript sequences (only coding sequence (CDS) region).

>In-frame_ESR1_ENST00000440973_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_1908nt
ATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGCAG
CTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTAC
GAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGC
TCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTTTC
CTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTAC
AGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAG
GAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAG
AGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGC
CGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAG
AGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGC
TCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAG
TATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGG
GCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTG
GTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAG
AAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTT
CCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTT
GATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGC
CGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAA
CTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCT
CTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATT
TACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAA

>In-frame_ESR1_ENST00000338799_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_1908nt
ATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGCAG
CTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTAC
GAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGC
TCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTTTC
CTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTAC
AGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAG
GAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAG
AGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGC
CGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAG
AGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGC
TCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAG
TATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGG
GCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTG
GTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAG
AAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTT
CCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTT
GATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGC
CGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAA
CTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCT
CTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATT
TACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAA

>In-frame_ESR1_ENST00000443427_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_1908nt
ATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGCAG
CTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTAC
GAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGC
TCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTTTC
CTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTAC
AGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAG
GAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAG
AGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGC
CGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAG
AGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGC
TCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAG
TATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGG
GCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTG
GTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAG
AAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTT
CCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTT
GATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGC
CGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAA
CTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCT
CTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATT
TACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAA

>In-frame_ESR1_ENST00000206249_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_1908nt
ATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGCAG
CTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTAC
GAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGC
TCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTTTC
CTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTAC
AGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAG
GAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAG
AGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGC
CGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAG
AGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGC
TCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAG
TATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGG
GCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTG
GTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAG
AAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTT
CCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTT
GATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGC
CGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAA
CTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCT
CTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATT
TACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAA

>In-frame_ESR1_ENST00000427531_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_1389nt
ATGGCTATGGAATCTGCCAAGGAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAG
GGCTGCAAGGCCTTCTTCAAGAGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGG
AGGAAGAGCTGCCAGGCCTGCCGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGA
ATGTTGAAACACAAGCGCCAGAGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCA
AGCCCGCTCATGATCAAACGCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAG
CCCCCGATACTCTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAG
CTGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATC
AAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTA
ACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAG
ATCACTCAGCTCTTTGGGGTTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTT
GCAAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGA
GAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTC
TATGGAGCCAAAGATATTGAACTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGC
ATGGCAAAGACCCACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCC
AGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGAT

* Fusion transcript sequences (Full-length transcript).

>In-frame_ESR1_ENST00000440973_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_2671nt
ATGGTCATAACAGCCTCCTGTCTACCGACTCAGAACGGATTTTACCAAAACTGAAAATGCAGGCTCCATGCTCAGAAGCTCTTTAACAGG
CTCGAAAGGTCCATGCTCCTTTCTCCTGCCCATTCTATAGCATAAGAAGACAGTCTCTGAGTGATAATCTTCTCTTCAAGAAGAAGAAAA
CTAGGAAGGAGTAAGCACAAAGATCTCTTCACATTCTCCGGGACTGCGGTACCAAATATCAGCACAGCACTTCTTGAAAAAGGATGTAGA
TTTTAATCTGAACTTTGAACCATCACTGAGGTGGCCCGCCGGTTTCTGAGCCTTCTGCCCTGCGGGGACACGGTCTGCACCCTGCCCGCG
GCCACGGACCATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAA
CCGTCCGCAGCTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGG
CGCCGCCTACGAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGC
GGCGTTCGGCTCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCT
GTCGCCTTTCCTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCC
GGCATTCTACAGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGA
ATCTGCCAAGGAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGC
CTTCTTCAAGAGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTG
CCAGGCCTGCCGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACA
CAAGCGCCAGAGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCAT
GATCAAACGCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACT
CTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACAT
GATCAACTGGGCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGC
TTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGT
TCCTCTTAAGAAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCT
CTTTGGGGTTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGC
TGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAG
TAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAA
AGATATTGAACTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGAC
CCACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGC
TGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGA
AACAGAACAAGTTAAAGGCTTGTTCTAAGTGGACAAGGCTCTCACAGGACCCGATGCAGACTCCTGAAACAGACTACTCTTTGCCTTTTT
GCTGCAGTTGGAGAAGAAACTGAATTTGAAAAATGTCTGTTATGCAATGCTGGAGACATGGTGAAATAGGCCAAAGATTTCTTCTTCGTT
CAAGATGAATTCTGTTCACAGTGGAGTATGGTGTTCGGCAAAAGGACCTCCACCAAGACTGAAAGAAACTAATTTATTTCTGTTTCTGTG
GAGTTTCCATTATTTCTACTGCTTACACTTTAGAATGTTTATTTTATGGGGACTAAGGGATTAGGAGTGTGAACTAAAAGGTAACATTTT

>In-frame_ESR1_ENST00000338799_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_3761nt
CTAGAGCATGGGTGGCCATTGTTGACCTACAGGAGGCACCACTGTCACCAACAAAGGGTAACAGTCTTTCTTTTCAATATTTATTTATAT
CCAGTATTTATTTTCAATACTGACTATGGAGAGAGCTCTCCTGTGCTCAAACACTGCAATACTGGGGGTCTTTCAAAGCACAAAAACATA
TATTTGCATGATGGCATCATTAACATTTTTATGGCTTTCTATTTCTTTTTTGTACTGGTCTCAAGAGCCACTCATAAATCTCTCAGTAAC
TGCATAGTGTCCCAGGGCCAGAGACCGGCCACTCCTGGCATTGTGATTAGAGTCATTTAATATCCAAGGTGGTGACTAATGTCTGGCAAC
AAAGCCTCCATTGGGTGTCATGTGTCCTGGGACCCTGAGCGTGGGCACTCTAGGAGCACCTCAGTATTGCGTGTTAGTACTATGGCCGAG
AGAATAGTTGAGAAAGTGGTCAAGAGGTGGATCCATGTGAACGCCACTGGGAAATGAGAGACCTCGTTCCCAATCACGGTCAGTGCAACT
CGAAAGCCTAAAATCAGTTTAAAACAAAGGTATCTACCTTTATCTTATGTTCATATCCTAGGCTTTTAATAATACGTATTTTTCACATGT
TTACAGAAAGCAGTCAACTGAGCTATTCATGGAAAGGTTTGTGGGTTTGGTTAACGAAGTGGAGGAGTATTACATTTCAGCTGGAAACAC
ATCCCTAGAATGCCAAAACATTTATTCCAAAGTCTGGTTTCCTGGTGCAATCGGAGGCATGGCAATGCCTCTGTTCAGAGACTGGGGGCT
AGGGCCAGTAAGGCATTTGATCCACATGTATCCCAGAAGGCTTTTATTGTTAAATTATATTCTTTCGGAAAAACCACCCATGTCCTATTT
TGTAAACTTGATATCCATACACTTTTGACTGGCATTCTATTTTAGCCGTAAGACTATGATTCACAGCAAGCCTGTTTTTCCTCTTGCTTG
GGGTGGCAGCAGAAAGCATAGGGTACTTTCCAGCCTCCAAGGGTAGGGGCAAAGGGGCTGGGGTTTCTCCTCCCCAGTACAGCTTTCTCT
GGCTGTGCCACACTGCTCCCTGTGAGCAGACAGCAAGTCTCCCCTCACTCCCCACTGCCATTCATCCAGCGCTGTGCAGTAGCCCAGCTG
CGTGTCTGCCGGGAGGGGCTGCCAAGTGCCCTGCCTACTGGCTGCTTCCCGAATCCCTGCCATTCCACGCACAAACACATCCACACACTC
TCTCTGCCTAGTTCACACACTGAGCCACTCGCACATGCGAGCACATTCCTTCCTTCCTTCTCACTCTCTCGGCCCTTGACTTCTACAAGC
CCATGGAACATTTCTGGAAAGACGTTCTTGATCCAGCAGGGTGGCCCGCCGGTTTCTGAGCCTTCTGCCCTGCGGGGACACGGTCTGCAC
CCTGCCCGCGGCCACGGACCATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGG
AGCCCCTGAACCGTCCGCAGCTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACT
ACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGT
CTGAGGCTGCGGCGTTCGGCTCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGC
CGCCGCAGCTGTCGCCTTTCCTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG
CCGGCCCGCCGGCATTCTACAGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTA
TGGCTATGGAATCTGCCAAGGAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGG
GCTGCAAGGCCTTCTTCAAGAGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGA
GGAAGAGCTGCCAGGCCTGCCGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAA
TGTTGAAACACAAGCGCCAGAGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAA
GCCCGCTCATGATCAAACGCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGC
CCCCGATACTCTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGC
TGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCA
AGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAA
CGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGA
TCACTCAGCTCTTTGGGGTTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTG
CAAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAG
AGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCT
ATGGAGCCAAAGATATTGAACTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCA
TGGCAAAGACCCACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCA
GCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATC
TTGATACCGAAACAGAACAAGTTAAAGGCTTGTTCTAAGTGGACAAGGCTCTCACAGGACCCGATGCAGACTCCTGAAACAGACTACTCT
TTGCCTTTTTGCTGCAGTTGGAGAAGAAACTGAATTTGAAAAATGTCTGTTATGCAATGCTGGAGACATGGTGAAATAGGCCAAAGATTT
CTTCTTCGTTCAAGATGAATTCTGTTCACAGTGGAGTATGGTGTTCGGCAAAAGGACCTCCACCAAGACTGAAAGAAACTAATTTATTTC
TGTTTCTGTGGAGTTTCCATTATTTCTACTGCTTACACTTTAGAATGTTTATTTTATGGGGACTAAGGGATTAGGAGTGTGAACTAAAAG

>In-frame_ESR1_ENST00000443427_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_2562nt
GACAAACAGAGATATATCGGAGTCTGGCACGGGGCACATAAGGCAGCACATTAGAGAAAGCCGGCCCCTGGATCCGTCTTTCGCGTTTAT
TTTAAGCCCAGTCTTCCCTGGGCCACCTTTAGCAGATCCTCGTGCGCCCCCGCCCCCTGGCCGTGAAACTCAGCCTCTATCCAGCAGCGA
CGACAAGTAAAGTGGCCCGCCGGTTTCTGAGCCTTCTGCCCTGCGGGGACACGGTCTGCACCCTGCCCGCGGCCACGGACCATGACCATG
ACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGCAGCTCAAGATC
CCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTACGAGTTCAAC
GCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGCTCCAACGGC
CTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTTTCCTGCAGCCC
CACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTACAGGCCAAAT
TCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAGGAGACTCGC
TACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAGAGAAGTATT
CAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGCCGGCTCCGT
AAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAGAGAGATGAT
GGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGCTCTAAGAAG
AACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAGTATGATCCT
ACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGGGCGAAGAGG
GTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAAC
GTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATAT
ACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTCCCGTTGTG
GTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTTGATGCAGTC
CCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAG
TTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCCT
GAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCTCTATCTCAC
CAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTG
GTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGC
TTGTTCTAAGTGGACAAGGCTCTCACAGGACCCGATGCAGACTCCTGAAACAGACTACTCTTTGCCTTTTTGCTGCAGTTGGAGAAGAAA
CTGAATTTGAAAAATGTCTGTTATGCAATGCTGGAGACATGGTGAAATAGGCCAAAGATTTCTTCTTCGTTCAAGATGAATTCTGTTCAC
AGTGGAGTATGGTGTTCGGCAAAAGGACCTCCACCAAGACTGAAAGAAACTAATTTATTTCTGTTTCTGTGGAGTTTCCATTATTTCTAC
TGCTTACACTTTAGAATGTTTATTTTATGGGGACTAAGGGATTAGGAGTGTGAACTAAAAGGTAACATTTTCCACTCTCAAGTTTTCTAC

>In-frame_ESR1_ENST00000206249_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_2663nt
GAGTTGTGCCTGGAGTGATGTTTAAGCCAATGTCAGGGCAAGGCAACAGTCCCTGGCCGTCCTCCAGCACCTTTGTAATGCATATGAGCT
CGGGAGACCAGTACTTAAAGTTGGAGGCCCGGGAGCCCAGGAGCTGGCGGAGGGCGTTCGTCCTGGGACTGCACTTGCTCCCGTCGGGTC
GCCCGGCTTCACCGGACCCGCAGGCTCCCGGGGCAGGGCCGGGGCCAGAGCTCGCGTGTCGGCGGGACATGCGCTGCGTCGCCTCTAACC
TCGGGCTGTGCTCTTTTTCCAGGTGGCCCGCCGGTTTCTGAGCCTTCTGCCCTGCGGGGACACGGTCTGCACCCTGCCCGCGGCCACGGA
CCATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTGGAGCCCCTGAACCGTCCGC
AGCTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGCAGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCT
ACGAGTTCAACGCCGCGGCCGCCGCCAACGCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCG
GCTCCAACGGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCGCAGCTGTCGCCTT
TCCTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGGCCGGCCCGCCGGCATTCT
ACAGGCCAAATTCAGATAATCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCA
AGGAGACTCGCTACTGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCA
AGAGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCT
GCCGGCTCCGTAAATGCTACGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCC
AGAGAGATGATGGGGAGGGCAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAAC
GCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCG
AGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACT
GGGCGAAGAGGGTGCCAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCT
TGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTA
AGAAAGAATATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGG
TTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCT
TTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAA
GCCGATTCCAGTTCCTGTATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTG
AACTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTT
CTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCA
TTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAAC
AAGTTAAAGGCTTGTTCTAAGTGGACAAGGCTCTCACAGGACCCGATGCAGACTCCTGAAACAGACTACTCTTTGCCTTTTTGCTGCAGT
TGGAGAAGAAACTGAATTTGAAAAATGTCTGTTATGCAATGCTGGAGACATGGTGAAATAGGCCAAAGATTTCTTCTTCGTTCAAGATGA
ATTCTGTTCACAGTGGAGTATGGTGTTCGGCAAAAGGACCTCCACCAAGACTGAAAGAAACTAATTTATTTCTGTTTCTGTGGAGTTTCC
ATTATTTCTACTGCTTACACTTTAGAATGTTTATTTTATGGGGACTAAGGGATTAGGAGTGTGAACTAAAAGGTAACATTTTCCACTCTC

>In-frame_ESR1_ENST00000427531_chr6_152265643_+_MTHFD1L_ENST00000367321_chr6_151330955_+_2014nt
GCTTAAAAATAATCTCCTGCCAGCCCAGTGACAAGCCTGTCCCACCCGGGGAGAATGCCCCGGAGTGGCGTGCGGGTCAGCCAGGGTCTG
CGCCTCGCAGCCACTGTGGAAGGAGCGCGGCCGGTCCAGGACACAGGAGACCACTTTGTGACTTCAATGGCGAAGGCCAAATTCAGATAA
TCGACGCCAGGGTGGCAGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAGGAGACTCGCTACTGTGC
AGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCCTTCTTCAAGAGAAGTATTCAAGGACA
TAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGATAAAAACAGGAGGAAGAGCTGCCAGGCCTGCCGGCTCCGTAAATGCTA
CGAAGTGGGAATGATGAAAGGTGGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAGAGAGATGATGGGGAGGG
CAGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGCTCTAAGAAGAACAGCCT
GGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCGATACTCTATTCCGAGTATGATCCTACCAGACC
CTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGT
GACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGT
GTTAGTGGCAACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGA
GAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTCCCGTTGTGGTGGCTCT
GAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTA
TCACTGGTCCGTTGGTGGAAAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTA
TGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCCTGAGGCACA
AGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACCCACCTTTCTCTATCTCACCAACCTGA
CAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAAC
GATGAGCACCATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGCTTGTTCTA
AGTGGACAAGGCTCTCACAGGACCCGATGCAGACTCCTGAAACAGACTACTCTTTGCCTTTTTGCTGCAGTTGGAGAAGAAACTGAATTT
GAAAAATGTCTGTTATGCAATGCTGGAGACATGGTGAAATAGGCCAAAGATTTCTTCTTCGTTCAAGATGAATTCTGTTCACAGTGGAGT
ATGGTGTTCGGCAAAAGGACCTCCACCAAGACTGAAAGAAACTAATTTATTTCTGTTTCTGTGGAGTTTCCATTATTTCTACTGCTTACA
CTTTAGAATGTTTATTTTATGGGGACTAAGGGATTAGGAGTGTGAACTAAAAGGTAACATTTTCCACTCTCAAGTTTTCTACTTTGTCTT

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FusionGenePPI for ESR1_MTHFD1L

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page
.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene	Hgene's interactors	Tgene	Tgene's interactors
ESR1	PPARGC1B, SMARCA4, DDX54, DNTTIP2, NCOA6, BRCA1, NCOA4, RBM39, SRC, NELFB, GADD45G, GADD45A, CTNNB1, PTEN, SAFB, ZBTB16, XBP1, PHB2, PTMA, SMARCD1, TDG, SAFB2, AHR, EP300, MTA1, MNAT1, CCNH, SMARCE1, SMARCA2, FKBP5, FKBP4, HSP90AA1, HSPA4, PPID, PTGES3, YWHAQ, CDC25B, MGMT, PELP1, TRIP4, NCOA1, CREBBP, PRDM2, KDM5A, PRMT1, ESR1, NR2C2, POU2F1, POU2F2, STAT5A, RBFOX2, DAP3, NR2F1, MAPK1, TP53, MDM2, CDK7, GTF2H1, NCOA2, MVP, ESR2, NRIP1, CEBPA, CEBPB, CCND1, STAT3, SVIL, NR0B2, IGF1R, PAK6, POU4F1, POU4F2, REXO4, NR2C1, AKAP13, TBP, PPARGC1A, NCOA3, SHC1, TADA3, CAV1, RNF4, PIAS2, TRIM24, NR2F6, ISL1, NCOA7, FOXO1, FOXO4, PIK3R1, BAG1, NCOR1, JUN, ZNF398, PRMT2, SMAD2, MED12, MED1, MED14, MED23, MED24, MED16, MED6, MED20, MED10, MED17, MED7, MED21, MED13, CCNC, CDK8, RLIM, PDLIM1, LDB1, TRAM1, TRIM25, KAT5, MUC1, UBE3A, STUB1, HSPA8, HDAC7, HDAC4, HDAC5, HDAC9, FLII, SP1, SIN3A, KLF5, SMYD3, MYOD1, TOP2B, PRKDC, PARP1, XRCC5, XRCC6, MEN1, SP3, MTA2, RELA, MYC, RBBP7, RBBP4, AHRR, ING1, HDAC1, LMO4, LCOR, TAF1A, TAF1B, SETD7, SP2, MSH2, PGC, CDKN1A, IRS1, IRS2, CITED1, GRIP1, ATAD2, RPS6KA1, GTF2B, NCOR2, LRIF1, HDAC3, PHB, KAT6A, FOS, HDAC2, KDM1A, GSN, ACTB, NPM1, ACTC1, RPL18, DDX5, RPLP0, RPL7A, MYL6, RPL7, RPS4X, MYO1C, DDX3X, RPS9, RPS8, ACTR3, ACTR2, POLR2A, MBD2, RBBP6, SMAD3, SMURF1, SGK3, BARD1, ACTN4, OTUB1, DUT, NPPA, MTCH2, PNRC2, KIF1A, KMT2D, RBBP5, ASH2L, WDR5, AKT1, TUBB1, TUBA1A, MED25, PSMC5, RPS6KA3, AKT2, DDX17, TRRAP, MPG, BCAR1, PIK3R2, ERBB2, SOS2, FHL2, UBE2I, PIAS1, CUEDC2, SKIL, SKI, KDM4B, ARHGDIA, SIRT1, PRKCZ, TFF1, CALM1, BLOC1S1, EHMT2, TAF2, PGR, XPO1, RAN, E2F1, RABGEF1, TAB2, UIMC1, CBLL1, PPP1CC, CDK11B, SPOP, CUL3, COPS5, ANIB1, ARID5A, TRAF6, RBCK1, TBL1XR1, SIN3B, RBM23, ANP32A, CHUK, BCL3, CRIPAK, TSC2, EGFR, MYLK2, ARG1, DSG1, HIF1A, CLTCL1, HPCAL1, APOD, SERPINH1, BUD13, AKAP8, ATAD3A, BCLAF1, ELMSAN1, CAPRIN1, CLTC, DDX18, DDX21, DDX27, DHX15, DHX9, DKC1, EWSR1, FLNA, FTSJ3, FUS, GNL3, GTPBP4, HNRNPA3, HNRNPF, HNRNPK, HNRNPM, HNRNPR, HNRNPA1, HNRNPA1L2, ILF2, JUP, MKI67, MYBBP1A, MYH9, MYO1B, NAT10, NCL, NOP56, NUMA1, PABPC1, PGAM5, PTPLAD1, RBM14, RBMX, RPL10, RPL13, RPL13A, RPL14, RPL17, RPL18A, RPL19, RPL22, RPL24, RPL27A, RPL3, RPL36AL, RPL36A, RPL37A, RPL4, RPL6, RPL8, RPN1, RPN2, RPS14, RPS16, RPS2, RPS23, RPS26, RPS3A, RPS6, RRP12, RSL1D1, SRSF1, SRSF2, SRSF7, SLC25A5, SRRM1, TF, TFRC, THRAP3, TOP1, TOP2A, TRIP12, UBTF, U2AF2, ACTN1, AHNAK, AIFM1, ALKBH7, AP2M1, ARFGAP2, ARSB, ATP5B, ATP9A, BRI3BP, CAPZB, CHD4, LUC7L3, YBX3, DBN1, DDX50, DECR1, DEK, DHX30, DNAH2, DNAJB11, EFTUD2, EIF4A1, EIF4G1, EIF4G3, EP400, EPB41L5, FLNB, FMR1, G3BP1, G3BP2, GNB1, GNB2, GNB4, GNL2, GOLGA2, HNRNPA0, HNRNPA2B1, HNRNPAB, LYAR, MRPS9, MTDH, MYH14, MYO6, NOP2, NOP58, GLYR1, NUP153, POP1, PPP1CA, PRPF4B, PSIP1, RBM28, RPL10A, RPL11, RPL23A, RPL36, RPL5, RPL9, RPS12, RPS13, RPS15A, RPS20, RPS24, RPS3, RPSA, RUVBL1, RUVBL2, SCYL2, SRSF6, SHOC2, SNRPD1, SPTAN1, SPTBN1, SRPK1, SRPK2, SRRM2, STAU1, SUPT16H, TBL2, TCOF1, UBAP2L, UNC45A, UPF1, UTP3, WDR36, YBX1, YTHDF2, ANXA2, DDX3Y, THEM6, EMD, EPPK1, EXOSC10, H2AFX, HSPA1L, SYNCRIP, GAPDH, TECR, KHDRBS1, LGALS7, MAPKAPK2, MYL6B, NCCRP1, POF1B, PLEC, RPL29, RRP1B, RPL12, RPL28, RPL21, RPS18, RDX, SRSF5, S100A7, SERPINB4, SFN, SEC61B, SF3B1, SRSF3, S100A8, U2SURP, WDR18, VPS13D, ZNF512B, SRA1, NR1H4, BTF3, FHL1, VAV3, PPP5C, HEXIM1, CCNT1, PAK1, RAC3, SLC25A20, EZH2, TSC1, PAGR1, MMS19, CHD9, BCAS2, PIK3CA, ESRRA, FKBPL, ZDHHC21, ZDHHC7, KRT1, CEP290, HSPB1, PRDX2, NME2, PPIA, PFN1, CBR1, GSTM3, RNF31, RANBP9, WIPI1, NSD1, ZNF131, PSMB9, POLR2B, POLR2C, POLR2D, POLR2E, POLR2F, POLR2G, POLR2H, POLR2I, POLR2L, POLR2J, POLR2K, TTC5, ASCC1, SKP2, SKP1, CUL1, RBX1, CDK2, SMYD2, SUV39H1, WHSC1, SUV420H1, ANP32B, SND1, SFPQ, PQBP1, ALYREF, GNB2L1, CCT2, TCERG1, NUDT21, TOPBP1, CIRBP, DARS, DDX1, EEF2, EIF5A, TUBB, EPRS, FARSA, SEPT8, SERBP1, PRPF19, PDIA3, HIST1H2BD, H3F3A, H3F3B, HDLBP, HNRNPC, HNRNPD, ILF3, KARS, KRT8, KRT10, KRT18, KRT19, MARS, MTHFD1, NAP1L4, SEPT2, NONO, NSF, P4HB, PA2G4, RTCB, LARS, PPIB, SARS2, PPP2R1A, PSPC1, PRKCSH, PSMD1, SNX6, SET, SHMT2, HSP90B1, VCP, SMC1A, HIST2H2AC, HIST1H2BG, HIST1H2BF, HIST1H2BE, HIST1H2BI, HIST1H2BC, GRWD1, SARNP, TUBA1C, FBXO18, YARS, FUBP1, EIF2S2, SMC3, MED27, EIF4A3, BCAS3, DYX1C1, GNAI1, GNAI2, GNAI3, SMARCD3, ARID1A, SUPT6H, RNF40, FOXK2, ANGPTL4, BAP1, CXCL1, EPSTI1, GLCE, KLK5, KLK9, PSMC3IP, SERPINB5, WHSC1L1, CFL1, CSRP1, HBP1, UBE4B, RAD51, TUBG1, HECTD1, PPP2R3A, SASH1, UBE3C, USP16, SUZ12, NDC80, ZNF182, TAB3, HSPD1, TXNRD1, PATZ1, PPP2CA, PPP2CB, TRAF3, TBK1, IKBKE, FBXO45, TTK, CHD6, MPRIP, SNRPN, TMOD3, AGFG1, EIF6, GCN1L1, CORO1C, RAD50, APITD1-CORT, PRPF8, CAT, AP2A2, STOM, MED9, PRR12, BCOR, YTHDF1, HOXD9, HOXC10, NFYA, E2F6, HNRNPH2, FIP1L1, TIA1, SMC2, NCAPG, NCAPH2, SMC4, NCAPD2, NCAPH, NCAPD3, NCAPG2, DNMT3L, ARNT, VPRBP, LATS1, DCAF13, PPP1R14C, GBP1, PGK2, SIGLEC10, PNMT, CNDP1, ERBB4, RPTOR, MTOR, WBP2, FOXA1, HSPA1A, HSPA2, HSPA5, HSPA6, HSPA9, HSP90AB1, HSPH1, HSPA4L, DNAJA2, DNAJA3, DNAJB1, DNAJB4, DNAJB6, DNAJC7, DNAJC9, DNAJC10, HSPB8, HSPE1, PBX1, CARM1	MTHFD1L	CASP4, MAGED1, WRAP73, HGS, USP50, SIRT7, CUL3, COPS5, ADRB2, HECW2, ASB12, BPHL, CNN1, CNN3, ISOC1, TAGLN2, PAPOLA, PAPOLB, XPNPEP1, NTRK1, CEP152, NPM1, LRPPRC, NFATC1, MTMR7, CYLD

- Retained PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Still interaction with
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000206249	+	4	8	35_174	365	596	DDX5%3B self-association
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000338799	+	5	9	35_174	365	596	DDX5%3B self-association
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000440973	+	6	10	35_174	365	596	DDX5%3B self-association
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000443427	+	5	9	35_174	365	596	DDX5%3B self-association

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000206249	+	4	8	262_595	365	596	AKAP13
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000338799	+	5	9	262_595	365	596	AKAP13
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000440973	+	6	10	262_595	365	596	AKAP13
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000443427	+	5	9	262_595	365	596	AKAP13
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000456483	+	1	8	262_595	-73	484	AKAP13
Hgene	ESR1	chr6:152265643	chr6:151330955	ENST00000456483	+	1	8	35_174	-73	484	DDX5%3B self-association

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

RelatedDrugs for ESR1_MTHFD1L

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.0 2018-04-02)

Partner	Gene	UniProtAcc	DrugBank ID	Drug name	Drug activity	Drug type	Drug status
Hgene	ESR1	P03372	DB00286	Conjugated estrogens	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00304	Desogestrel	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00648	Mitotane	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00655	Estrone	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00675	Tamoxifen	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00841	Dobutamine	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB00977	Ethinyl Estradiol	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB01406	Danazol	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB01431	Allylestrenol	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB06710	Methyltestosterone	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB09369	Polyestradiol phosphate	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB09371	Norethynodrel	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB11071	Phenyl salicylate	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB11619	Gestrinone	Estrogen receptor	small molecule	approved
Hgene	ESR1	P03372	DB01185	Fluoxymesterone	Estrogen receptor	small molecule	approved\|illicit
Hgene	ESR1	P03372	DB00255	Diethylstilbestrol	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00294	Etonogestrel	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00367	Levonorgestrel	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00481	Raloxifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00539	Toremifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00603	Medroxyprogesterone acetate	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00624	Testosterone	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00882	Clomifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00890	Dienestrol	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00947	Fulvestrant	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB00957	Norgestimate	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB01196	Estramustine	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB01593	Zinc	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB04930	Permethrin	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB04938	Ospemifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB06202	Lasofoxifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB06401	Bazedoxifene	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB09070	Tibolone	Estrogen receptor	small molecule	approved\|investigational
Hgene	ESR1	P03372	DB01708	Prasterone	Estrogen receptor	small molecule	approved\|investigational\|nutraceutical
Hgene	ESR1	P03372	DB00783	Estradiol	Estrogen receptor	small molecule	approved\|investigational\|vet_approved
Hgene	ESR1	P03372	DB04573	Estriol	Estrogen receptor	small molecule	approved\|investigational\|vet_approved
Hgene	ESR1	P03372	DB01108	Trilostane	Estrogen receptor	small molecule	approved\|investigational\|vet_approved\|withdrawn
Hgene	ESR1	P03372	DB01065	Melatonin	Estrogen receptor	small molecule	approved\|nutraceutical\|vet_approved
Hgene	ESR1	P03372	DB00396	Progesterone	Estrogen receptor	small molecule	approved\|vet_approved
Hgene	ESR1	P03372	DB01183	Naloxone	Estrogen receptor	small molecule	approved\|vet_approved
Hgene	ESR1	P03372	DB00431	Lindane	Estrogen receptor	small molecule	approved\|withdrawn
Hgene	ESR1	P03372	DB00756	Hexachlorophene	Estrogen receptor	small molecule	approved\|withdrawn
Hgene	ESR1	P03372	DB04824	Phenolphthalein	Estrogen receptor	small molecule	approved\|withdrawn

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RelatedDiseases for ESR1_MTHFD1L

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	ESR1	C1458155	Mammary Neoplasms	11	CTD_human
Hgene	ESR1	C0005586	Bipolar Disorder	5	PSYGENET
Hgene	ESR1	C0011570	Mental Depression	5	PSYGENET
Hgene	ESR1	C0011581	Depressive disorder	5	PSYGENET
Hgene	ESR1	C0036341	Schizophrenia	5	PSYGENET
Hgene	ESR1	C0525045	Mood Disorders	4	PSYGENET
Hgene	ESR1	C0001418	Adenocarcinoma	2	CTD_human
Hgene	ESR1	C0021364	Male infertility	2	CTD_human
Hgene	ESR1	C0024121	Lung Neoplasms	2	CTD_human
Hgene	ESR1	C0024667	Animal Mammary Neoplasms	2	CTD_human
Hgene	ESR1	C0025500	Mesothelioma	2	CTD_human
Hgene	ESR1	C0033578	Prostatic Neoplasms	2	CTD_human
Hgene	ESR1	C1956346	Coronary Artery Disease	2	CTD_human
Hgene	ESR1	C3809250	ESTROGEN RESISTANCE	2	ORPHANET;UNIPROT
Hgene	ESR1	C0002395	Alzheimer's Disease	1	CTD_human
Hgene	ESR1	C0004114	Astrocytoma	1	CTD_human
Hgene	ESR1	C0004153	Atherosclerosis	1	CTD_human
Hgene	ESR1	C0005695	Bladder Neoplasm	1	CTD_human
Hgene	ESR1	C0005944	Metabolic Bone Disorder	1	CTD_human
Hgene	ESR1	C0007621	Neoplastic Cell Transformation	1	CTD_human
Hgene	ESR1	C0014170	Endometrial Neoplasms	1	CTD_human
Hgene	ESR1	C0015799	Feminization	1	CTD_human
Hgene	ESR1	C0017658	Glomerulonephritis	1	CTD_human
Hgene	ESR1	C0021361	Female infertility	1	CTD_human
Hgene	ESR1	C0023267	Fibroid Tumor	1	CTD_human
Hgene	ESR1	C0023418	leukemia	1	CTD_human
Hgene	ESR1	C0023903	Liver neoplasms	1	CTD_human
Hgene	ESR1	C0024668	Mammary Neoplasms, Experimental	1	CTD_human
Hgene	ESR1	C0024809	Marijuana Abuse	1	PSYGENET
Hgene	ESR1	C0026858	Musculoskeletal Pain	1	CTD_human
Hgene	ESR1	C0027051	Myocardial Infarction	1	CTD_human
Hgene	ESR1	C0027627	Neoplasm Metastasis	1	CTD_human
Hgene	ESR1	C0027643	Neoplasm Recurrence, Local	1	CTD_human
Hgene	ESR1	C0028754	Obesity	1	CTD_human
Hgene	ESR1	C0028960	Oligospermia	1	CTD_human
Hgene	ESR1	C0033941	Psychoses, Substance-Induced	1	CTD_human
Hgene	ESR1	C0036939	Shared Paranoid Disorder	1	PSYGENET
Hgene	ESR1	C0149931	Migraine Disorders	1	CTD_human
Hgene	ESR1	C0282612	Prostatic Intraepithelial Neoplasias	1	CTD_human
Hgene	ESR1	C1720887	Female Urogenital Diseases	1	CTD_human
Hgene	ESR1	C2239176	Liver carcinoma	1	CTD_human

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Fusion gene ID: 12093

FusionGeneSummary for ESR1_MTHFD1L

FusionProtFeatures for ESR1_MTHFD1L

FusionGeneSequence for ESR1_MTHFD1L

FusionGenePPI for ESR1_MTHFD1L

RelatedDrugs for ESR1_MTHFD1L

RelatedDiseases for ESR1_MTHFD1L