About FusionGDB

FusionGDB is the Fusion Gene annotation DataBase, aiming to provide a resource or reference for functional annotation of fusion genes in cancer for better therapeutic targets. We first collected 48 117 FGs across pan-cancer from three representative fusion gene resources: the improved database of chimeric transcripts and RNA-seq data (ChiTaRS 3.1), an integrative resource for cancer-associated transcript fusions (TumorFusions), and The Cancer Genome Atlas (TCGA) fusions by Gao et al. For these ~48K FGs, we performed functional annotations including gene assessment across pan-cancer fusion genes, open reading frame (ORF) assignment, and protein domain retention search based on multiple isoform gene structure with multiple break points and finally provided the fusion transcript and amino acid sequences for each break point and gene isoforms. For each fusion partner gene, the user can access multiple annotations such as gene summary, assessment scores of each gene in pan-cancer, biological process gene ontologies, functional description, retention information of 39 protein features and protein-protein interaction (PPI), related drugs and diseases through six categories. Among ~44K FGs checked ORFs, there were ~ 10K in-frame and ~11K frame-shift FGs. Of these, we have identified 331, 303, 840, and 667 in-frame FGs retaining kinase domain, DNA-binding domain, oncogene domains, and epifactor domains in fusion proteins. Furthermore, we identified 896 and 118 in-frame FGs not-retained their functional domains of tumor suppressor genes and DNA damage repair genes, respectively. On the other hand, we identified 6863 FGs retaining their functional domains, but lost the function due to the frame-shift.

Updates:
- We developed a second version of FusionGDB2.0, which has a intensive functional annotation on ~ 110K human fusion genes with enhanced contents. Please visit https://compbio.uth.edu/FusionGDB2.
- You can download the fusion transcript sequence with the breakpoint information from the download page. For the fusion CDS, and amino acid sequences, we just provide the total length information. (12/06/2020)
- Recently, we have found an error in the process of translating the fusion CDS sequence to amino acid sequence. We corrected errors and uploaded those in both search page and download page. Please use this new fusion amino acid sequeces. (01/09/20)