	FusionGeneSummary
	FusionProtFeature
	FusionGeneSequence
	FusionGenePPI
	RelatedDrugs
	RelatedDiseases

Fusion gene ID: 10159

FusionGeneSummary for DMTN_PKN2

Fusion gene summary

Fusion gene information	Fusion gene name: DMTN_PKN2
	Fusion gene ID: 10159
		Hgene	Tgene
	Gene symbol	DMTN	PKN2
	Gene ID	2039	5586
	Gene name	dematin actin binding protein	protein kinase N2
	Synonyms	DMT\|EPB49	PAK2\|PRK2\|PRKCL2\|PRO2042\|Pak-2\|STK7
	Cytomap	8p21.3	1p22.2
	Type of gene	protein-coding	protein-coding
	Description	dematinerythrocyte membrane protein band 4.9 (dematin)	serine/threonine-protein kinase N2PKN gammacardiolipin-activated protein kinase Pak2protein kinase C-like 2protein-kinase C-related kinase 2
	Modification date	20180523	20180523
	UniProtAcc	Q08495	Q16513
	Ensembl transtripts involved in fusion gene	ENST00000381470, ENST00000432128, ENST00000443491, ENST00000517600, ENST00000523782, ENST00000265800, ENST00000415253, ENST00000358242, ENST00000523266, ENST00000519907,	ENST00000316005, ENST00000370521, ENST00000370505, ENST00000370513, ENST00000544045, ENST00000495119,
Fusion gene scores	* DoF score	4 X 4 X 4=64	4 X 2 X 3=24
	# samples	6	3
	** MAII score	log2(6/64*10)=-0.0931094043914815 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(3/24*10)=0.321928094887362 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context	PubMed: DMTN [Title/Abstract] AND PKN2 [Title/Abstract] AND fusion [Title/Abstract]
Functional or gene categories assigned by FusionGDB annotation

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	DMTN	GO:0032956	regulation of actin cytoskeleton organization	22927433
Hgene	DMTN	GO:0065003	protein-containing complex assembly	18347014
Hgene	DMTN	GO:0071320	cellular response to cAMP	22927433
Tgene	PKN2	GO:0006468	protein phosphorylation	17332740
Tgene	PKN2	GO:0010631	epithelial cell migration	21754995

Fusion gene information from three resources
(ChiTars (NAR, 2018), tumorfusions (NAR, 2018), Gao et al. (Cell, 2018))
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Data type	Source	Cancer type	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChiTaRS3.1			BP417512	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+

* LD: Li Ding group's fusion gene list
RV: Roel Verhaak group's fusion gene list
ChiTaRs fusion database

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
intron-intron	ENST00000381470	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000381470	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000381470	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000381470	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000381470	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000381470	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000432128	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000432128	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000432128	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000432128	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000432128	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000432128	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000443491	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000443491	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000443491	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000443491	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000443491	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000443491	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000517600	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000517600	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000517600	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000517600	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000517600	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000517600	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523782	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000523782	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523782	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523782	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523782	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523782	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000265800	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000265800	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000265800	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000265800	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000265800	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000265800	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000415253	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000415253	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000415253	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000415253	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000415253	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000415253	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000358242	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000358242	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000358242	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000358242	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000358242	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000358242	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523266	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000523266	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523266	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523266	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523266	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000523266	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000519907	ENST00000316005	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-3UTR	ENST00000519907	ENST00000370521	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000519907	ENST00000370505	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000519907	ENST00000370513	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000519907	ENST00000544045	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+
intron-intron	ENST00000519907	ENST00000495119	DMTN	chr8	21927698	-	PKN2	chr1	89299434	+

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FusionProtFeatures for DMTN_PKN2

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DMTN Q08495	PKN2 Q16513
Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation andstabilization. Its F-actin-bundling activity is reversiblyregulated upon its phosphorylation by the cAMP-dependent proteinkinase A (PKA). Binds to the erythrocyte membrane glucosetransporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches thespectrin-actin network to the erythrocytic plasma membrane. Playsa role in maintaining the functional integrity of PKA-activatederythrocyte shape and the membrane mechanical properties. Playsalso a role as a modulator of actin dynamics in fibroblasts; actsas a negative regulator of the RhoA activation pathway. Inplatelets, functions as a regulator of internal calciummobilization across the dense tubular system that affects plateletgranule secretion pathways and aggregation. Also required for theformation of a diverse set of cell protrusions, such as filopodiaand lamellipodia, necessary for platelet cell spreading, motilityand migration. Acts as a tumor suppressor and inhibits malignantcell transformation. {ECO:0000269\|PubMed:10565303,ECO:0000269\|PubMed:11856323, ECO:0000269\|PubMed:18347014,ECO:0000269\|PubMed:19241372, ECO:0000269\|PubMed:22927433,ECO:0000269\|PubMed:23355471}.	PKC-related serine/threonine-protein kinase and Rho/Raceffector protein that participates in specific signal transductionresponses in the cell. Plays a role in the regulation of cellcycle progression, actin cytoskeleton assembly, cell migration,cell adhesion, tumor cell invasion and transcription activationsignaling processes. Phosphorylates CTTN in hyaluronan-inducedastrocytes and hence decreases CTTN ability to associate withfilamentous actin. Phosphorylates HDAC5, therefore lead to impairHDAC5 import. Direct RhoA target required for the regulation ofthe maturation of primordial junctions into apical junctionformation in bronchial epithelial cells. Required for G2/M phasesof the cell cycle progression and abscission during cytokinesis ina ECT2-dependent manner. Stimulates FYN kinase activity that isrequired for establishment of skin cell-cell adhesion duringkeratinocytes differentiation. Regulates epithelial bladder cellsspeed and direction of movement during cell migration and tumorcell invasion. Inhibits Akt pro-survival-induced kinase activity.Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leadingto stimulation of HCV RNA replication. Involved in the negativeregulation of ciliogenesis (PubMed:27104747).{ECO:0000269\|PubMed:10226025, ECO:0000269\|PubMed:10926925,ECO:0000269\|PubMed:11777936, ECO:0000269\|PubMed:11781095,ECO:0000269\|PubMed:15364941, ECO:0000269\|PubMed:17332740,ECO:0000269\|PubMed:20188095, ECO:0000269\|PubMed:20974804,ECO:0000269\|PubMed:21754995, ECO:0000269\|PubMed:27104747,ECO:0000269\|PubMed:9121475}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
.

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

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FusionGeneSequence for DMTN_PKN2

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences.
(nt: nucleotides, aa: amino acids)

* Fusion amino acid sequences.

* Fusion transcript sequences (only coding sequence (CDS) region).

* Fusion transcript sequences (Full-length transcript).

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FusionGenePPI for DMTN_PKN2

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page
.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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RelatedDrugs for DMTN_PKN2

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.0 2018-04-02)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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RelatedDiseases for DMTN_PKN2

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

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Fusion gene ID: 10159

FusionGeneSummary for DMTN_PKN2

FusionProtFeatures for DMTN_PKN2

FusionGeneSequence for DMTN_PKN2

FusionGenePPI for DMTN_PKN2

RelatedDrugs for DMTN_PKN2

RelatedDiseases for DMTN_PKN2